Biosorption of arsenic in laboratory condition from municipal wastewaters treatment plants as well as Polyacrylic effluent in Isfahan
Subject Areas : Microbial Biotechnology
Neda
Heidari Khoii
1
(MS Student ,Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran.)
Monir
Doudi
2
(گروه میکروبیولوژی، واحد فلاورجان، دانشگاه آزاد اسلامی، ایرن)
Keywords: MIC, colony-PCR, wastewater, Biosorption, Arsenic resistant,
Abstract :
Pollution of the invironment with metals is a serious threatment to human health and the environments. The purpose of this study of arsenic biosorption was from several industrial effluents. In this analytical study samples were collected from two wastewater treatment plants of ShahinShahr (1) and Polyacrylic Company (2). Temprature, pH, BOD, COD, and arsenic were measured. Heterotrophic bacteria were counted and arsenic-resistant bacteria were isolated by PHG II medium and evaluated for resistance to several antibiotics. Agar diffusion method (MIC) and (MBC) were used bacterial determinationand for colony-PCR. The concertrations of arsenic in effluents (1) and (2) were 0.730 and 1.895 mg/L, respectively and the pH of both effluents was neutral. The effluent BOD and COD effluent (2)was within the standard range, but the BOD and COD effluent (1) was somewhat higher. The number of heterotrophic bacteria in effluent (1) was higher than effluent (2), but there was no significant difference between these two effluents. Arsenic-resistant isolates in both effluents were Bacillus syphenis OPL, Pseudomonas aeruginosa IAUF-11ZBK, Stenotrophomonasmaltophilia IR-Isfahan (G) and 5633. Pseudomonas aeruginosaZBK showed the highest sensitivity to antibiotics, which was able to biosorption of arsenic from municipal wastewater up to 1.4 mg/L in vitro.Pseudomonas aeruginosa strain ZBK had a good ability to biosorption of arsenic and low resistance to antibiotics. This strain is recommended for bioabsorption of arsenic from effluents.
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