Evaluation of antimicrobial potentiality and type-I polyketide synthase and nonribosomal peptide synthetase biosynthetic genes from some marine actinomycetes

Subject Areas : Microbial Biotechnology

Fatemeh shayesteh ¹ (Department of Fisheries, Faculty of Marine Science and Technology, University of Hormozgan, Bandar Abbas, Iran.)
Nur Syuhana Binti Zakaria ² (School of Bioscience and Biotechnology, Faculty of Science and Technology, University Kebangsaan Malaysia)
Gires Usup ³ (School of Environmental Science and Natural Resources, Faculty of Science and Technology, University Kebangsaan Malaysia)
Asmat Ahmad ⁴ (School of Bioscience and Biotechnology, Faculty of Science and Technology, University Kebangsaan Malaysia)

Received: 2020-04-05 Accepted : 2020-06-15 Published : 2020-05-21

Keywords: Malaysia, Actinomycetes, Antimicrobial Activity, marine bacteria, PKS-I and NRPS genes,

Abstract :

Background and Objectives: Actinomycetes are commonly known as exceptionally prolific source of secondary metabolites with diverse biological activities. The aim of this study was to identify some actinomycete isolates from Malaysia marine environment and evaluate for type-I polyketide synthase (PKS-I) and nonribosomal peptide synthetase (NRPS) genes as well as antimicrobial activity. Materials and Methods: Selected isolates were identified based on their morphology and molecular properties. PKS-I and NRPS genes were detected using specific primers and the potential of their antimicrobial activity was investigated by disc diffusion method.Results: The isolates varied morphologically on the basis of colony morphology, spore chain shape, aerial and substrate mycelium formation. Based on 16S rRNA gene sequences analysis, isolates Sdstm3k, Sdtm108 and Sdts4 were highly similar to Streptomyces sp. (95 %), whereas isolates Bvpd17e and SctgJI demonstrated highest similarity to Micrococcus sp. M2-19 (99 %) and Micrococcus leteus (95 %) respectively. While isolate Sdsb2k1a and Sdts46 were unidentified. The detection of PKS-I and NRPS genes revealed that only isolates SctgJI and Sdsb2k1a had both genes. Isolates Streptomyces sp. Sdst3k1 and Streptomyces sp. Sdts4 demonstrated the strongest and broadest spectrum of antimicrobial activity against 10 human pathogens tested. Conclusion: The present study indicated that actinomycetes isolated from marine environment in Malaysia can be a good source of the discovery of new bioactive compounds.

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