Effects of microbial fertilizers on the chlorophyll a fluorescence responses of tomato plants
Subject Areas :
Microbial Biotechnology
Saber Nezamivand Chegini
1
(
Department of Biology, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran
)
Mojtaba Jafarinia
2
(
Department of Biology, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran
)
Ali Akbar Ghotbi Ravandi
3
(
Department of Plant Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran
)
Received: 2022-03-19
Accepted : 2022-08-20
Published : 2022-09-06
Keywords:
Biofertilizer,
Photosynthesis,
Chlorophyll a fluorescence,
Microbial fertilizer,
Abstract :
Background & Objectives: Plant growth-promoting bacteria in rhizosphere improve plants’ growth in different ways. In this study, the effects of five growth promoting bacteria, in the form of three biofertilizers were investigated on the chlorophyll a fluorescence changes of tomato seedlings.Materials and Methods: The experiments were conducted as factorial based on a completely randomized design. The bacteria were Azotobacter chroococcum, Azospirillum lipoferum, Bacillus lentus, Pseudomonas putida and Acidithiobacillus thiooxidans; and biofertilizers were nitroxin, biosporphosphate and biosulfur. Chlorophyll a fluorescence changes were recorded using a Handy PEA device and analyzed by the JIP-test method.Results: The results showed that the use of Azotobacter chroococcum and Azospirillum lipoferum caused L band formation about 15 microseconds after light exposure, which revealed an improvement in the grouping and connectivity of antenna chlorophylls with photosynthetic reaction centers. Besides, the formation of the K band about 0.3 milliseconds after light exposure showed that the performance of the water-splitting complex was increased under the influence of Azotobacter chroococcum and Azospirillum lipoferum. The increase in electron transfer efficiency and the rate of reduction of the end electron acceptors in photosystem I were confirmed by the formation of J, H, and G bands in the presence of plant growth-promoting bacteria. Conclusion: The results of this study proved that the best photosynthetic performance in tomato plants including electron transfer between carriers of the photosynthetic electron transport chain occurred in the nitroxin bio-fertilizer containing Azotobacter chroococcum and Azospirillum lipoferum.
References:
Reference
Anli M, Baslam M, Tahiri A, Raklami A, Symanczik S, Boutasknit A, et al. Biofertilizers as Strategies to Improve Photosynthetic Apparatus, Growth, and Drought Stress Tolerance in the Date Palm. Front Plant Sci. 2020;11(October):1–27.
Itelima J, Wj B, MD S, Ia O, Oj E. A review : Biofertilizer - A key player in enhancing soil fertility and crop productivity. Microbiol Biotechnol Rep. 2018;2(1):22–8.
Mitter EK, Tosi M, Obregón D, Dunfield KE, Germida JJ. Rethinking Crop Nutrition in Times of Modern Microbiology: Innovative Biofertilizer Technologies. Front Sustain Food Syst. 2021;5(February):1–23.
de Souza R, Ambrosini A, Passaglia LMP. Plant growth-promoting bacteria as inoculants in agricultural soils. Genet Mol Biol. 2015;38(4):401–19.
Younesi H, Hassani SB, Ghotbi Ravandi AA, Soltani N. Plant Growth Promoting Potential of Phormidium sp. ISC108 on Seed Germination, Growth Indices and Photosynthetic Efficiency of Maize (Zea mays L.). J Phycol Res. 2019;3(2):375–85.
Al-Bdairi SHJ, Kamal JA. The Effect of Biofertilizer of Azolla, Phosphate and Nitrogen Fertilizers on some Growth Traits of Rice. IOP Conf Ser Earth Environ Sci. 2021;735(1).
Suma N, Srimathi P, Roopa VM. Original Research Article Influence of Biofertilizer pelleting on seed and seedling quality characteristics of Sesamum indicum At the time of germination count , ten normal seedlings were taken at random . The length between the collar and tip of the prim. 2014;3(6):591–4.
Ye L, Zhao X, Bao E, Li J, Zou Z, Cao K. Bio-organic fertilizer with reduced rates of chemical fertilization improves soil fertility and enhances tomato yield and quality. Sci Rep [Internet]. 2020;10(1):1–11. Available from: http://dx.doi.org/10.1038/s41598-019-56954-2
Zagorchev L, Atanasova A, Albanova I, Traianova A, Mladenov P, Kouzmanova M, et al. Functional characterization of the photosynthetic machinery in smicronix galls on the parasitic plant cuscuta campestris by jip-test. Cells. 2021;10(6).
Bagheenayat, N., Barzin, G., Jafarinia, M., Pishkar, L. and Entezari M. Investigation of the effects of salinity stress on the performance of photosynthetic electron transport chain in different species of Salvia probed by JIP test. J Plant Process Funct [Internet]. 2021;10(44): 77–92. Available from: http://jispp.iut.ac.ir/article-1-1464-en.html
Shahsavandi F, Eshghi S, Gharaghani A, Ghasemi-fasaei R, Jafarinia M. Scientia Horticulturae E ff ects of bicarbonate induced iron chlorosis on photosynthesis apparatus in grapevine. Sci Hortic (Amsterdam) [Internet]. 2020;270(December 2019):109427. Available from: https://doi.org/10.1016/j.scienta.2020.109427
Kalaji HM, Jajoo A, Oukarroum A, Brestic M. Chlorophyll a fluorescence as a tool to monitor physiological status of plants under abiotic stress conditions. Acta Physiol Plant. 2016;38(102):1–11.
Collins EJ, Bowyer C, Tsouza A, Chopra M. Tomatoes: An Extensive Review of the Associated Health Impacts of Tomatoes and Factors That Can Affect Their Cultivation. Biology (Basel). 2022;11(2).
Jafarinia M, Shariati M. Effects of salt stress on photosystem II of canola plant ( Barassica napus , L .) probing by chlorophyll a fluorescence measurements. Iran J Sci Technol. 2012; 71–6.
Das HK. Azotobacters as biofertilizer [Internet]. 1st ed. Vol. 108, Advances in Applied Microbiology. Elsevier Inc.; 2019. 1-43 p. Available from: http://dx.doi.org/10.1016/bs.aambs.2019.07.001
Sumbul A, Ansari RA, Rizvi R, Mahmood I. Azotobacter: A potential bio-fertilizer for soil and plant health management. Saudi J Biol Sci [Internet]. 2020;27(12):3634–40. Available from: https://doi.org/10.1016/j.sjbs.2020.08.004
Raffi MM, Charyulu PBBN. Azospirillum-biofertilizer for sustainable cereal crop production: Current status [Internet]. Recent Developments in Applied Microbiology and Biochemistry. Elsevier Inc.; 2021. 193-209 p. Available from: http://dx.doi.org/10.1016/B9 78-0-12-821406-0.00018-7
Suhameena B, Devi S, Gowri R, Kumar A. Utilization of Azospirillum as a Biofertilizer – An Overview. Int J Pharm Rev Res. 2020;62(22):141–5.
Kalaji HM, Oukarroum A, Alexandrov V, Kouzmanova M, Brestic M, Zivcak M, et al. Identification of nutrient deficiency in maize and tomato plants by in vivo chlorophyll a fluorescence measurements. Plant Physiol Biochem [Internet]. 2014 Aug [cited 2014 Aug 31];81(April):16–25. Available from: http://www.ncbi.nlm.nih.gov/pubmed/24811616
Chen W, Jia B, Chen J, Feng Y, Li Y, Chen M, et al. Effects of Different Planting Densities on Photosynthesis in Maize Determined via Prompt Fluorescence, Delayed Fluorescence and P700 Signals. Plants. 2021;10(276):1–17.
Rosa WS, Martins JPR, Rodrigues ES, de Almeida Rodrigues LC, Gontijo ABPL, Falqueto AR. Photosynthetic apparatus performance in function of the cytokinins used during the in vitro multiplication of Aechmea blanchetiana (Bromeliaceae). Plant Cell Tissue Organ Cult [Internet]. 2018;133(3):339–50. Available from: http://dx.doi.org/10.1007/s11240-018-1385-x
Zubek S, Turnau K, Tsimilli-Michael M, Strasser RJ. Response of endangered plant species to inoculation with arbuscular mycorrhizal fungi and soil bacteria. Mycorrhiza [Internet]. 2009 Feb [cited 2014 Sep 1];19(2):113–23. Available from: http://www.ncbi.nlm.nih.gov/pubmed/19011910
Khalilpoor M, Jafarinia M. Investigation the Effects of Salinity and Nitric Oxide on the Changes of Chlorophyll a Fluorescence in Oat ( Avena sativa L .) Plant Probed by JIP-Test. Iran J plant biol. 2017; 9(31): 87-98.
Motamed S, Jafarinia M, Kholdebarin B. Investigating the effects of drought stress on photosynthetic electron transport chain of two basil ( Ocimum basilicum L .) cultivars by m easuring “ Chlorophyll - a ” fluorescence. J Biodivers Environ Sci. 2015;7(1):564–71.
Gupta R. The oxygen-evolving complex: a super catalyst for life on earth, in response to abiotic stresses. Plant Signal Behav [Internet]. 2020;15(12). Available from: https://doi.org/10.1080/15592324.2020.1824721
Paul S, Neese F, Pantazis DA. Structural models of the biological oxygen-evolving complex: Achievements, insights, and challenges for biomimicry. Green Chem. 2017;19(10):2309–25.
Cham R, Ali S, Jafarinia M, Yasrebi J. South African Journal of Botany Physiological responses of Dracocephalum kotschyi Boiss . to drought stress and Bio-fertilizers. South African J Bot [Internet]. 2022;148:180–9. Available from: https://doi.org/10.1016/j.sajb.2022.04.008
Vitale L, Vitale E, Guercia G, Turano M, Arena C. Effects of different light quality and biofertilizers on structural and physiological traits of Spinach plants. Photosynthetica [Internet]. 2020;58(4):932–43. Available from: https://doi.org/10.32615/ps.2020.039
Dimitrova S, Paunov M, Pavlova B, Dankov K, Kouzmanova M, Velikova V, et al. Photosynthetic efficiency of two platanus orientalis l. Ecotypes exposed to moderately high temperature – jip-test analysis. Photosynthetica [Internet]. 2020;58(Special Issue):657–70. Available from: https://doi.org/10.32615/ps.2020.012
Basset GJ, Latimer S, Fatihi A, Soubeyrand E, Block A. Phylloquinone (Vitamin K1): Occurrence, Biosynthesis and Functions. Mini-Reviews Med Chem. 2017;17(12).
Corpas FJ, González-Gordo S, Palma JM. Nitric oxide and hydrogen sulfide modulate the NADPH-generating enzymatic system in higher plants. J Exp Bot. 2021;72(3):830–47.
_||_Reference
Anli M, Baslam M, Tahiri A, Raklami A, Symanczik S, Boutasknit A, et al. Biofertilizers as Strategies to Improve Photosynthetic Apparatus, Growth, and Drought Stress Tolerance in the Date Palm. Front Plant Sci. 2020;11(October):1–27.
Itelima J, Wj B, MD S, Ia O, Oj E. A review : Biofertilizer - A key player in enhancing soil fertility and crop productivity. Microbiol Biotechnol Rep. 2018;2(1):22–8.
Mitter EK, Tosi M, Obregón D, Dunfield KE, Germida JJ. Rethinking Crop Nutrition in Times of Modern Microbiology: Innovative Biofertilizer Technologies. Front Sustain Food Syst. 2021;5(February):1–23.
de Souza R, Ambrosini A, Passaglia LMP. Plant growth-promoting bacteria as inoculants in agricultural soils. Genet Mol Biol. 2015;38(4):401–19.
Younesi H, Hassani SB, Ghotbi Ravandi AA, Soltani N. Plant Growth Promoting Potential of Phormidium sp. ISC108 on Seed Germination, Growth Indices and Photosynthetic Efficiency of Maize (Zea mays L.). J Phycol Res. 2019;3(2):375–85.
Al-Bdairi SHJ, Kamal JA. The Effect of Biofertilizer of Azolla, Phosphate and Nitrogen Fertilizers on some Growth Traits of Rice. IOP Conf Ser Earth Environ Sci. 2021;735(1).
Suma N, Srimathi P, Roopa VM. Original Research Article Influence of Biofertilizer pelleting on seed and seedling quality characteristics of Sesamum indicum At the time of germination count , ten normal seedlings were taken at random . The length between the collar and tip of the prim. 2014;3(6):591–4.
Ye L, Zhao X, Bao E, Li J, Zou Z, Cao K. Bio-organic fertilizer with reduced rates of chemical fertilization improves soil fertility and enhances tomato yield and quality. Sci Rep [Internet]. 2020;10(1):1–11. Available from: http://dx.doi.org/10.1038/s41598-019-56954-2
Zagorchev L, Atanasova A, Albanova I, Traianova A, Mladenov P, Kouzmanova M, et al. Functional characterization of the photosynthetic machinery in smicronix galls on the parasitic plant cuscuta campestris by jip-test. Cells. 2021;10(6).
Bagheenayat, N., Barzin, G., Jafarinia, M., Pishkar, L. and Entezari M. Investigation of the effects of salinity stress on the performance of photosynthetic electron transport chain in different species of Salvia probed by JIP test. J Plant Process Funct [Internet]. 2021;10(44): 77–92. Available from: http://jispp.iut.ac.ir/article-1-1464-en.html
Shahsavandi F, Eshghi S, Gharaghani A, Ghasemi-fasaei R, Jafarinia M. Scientia Horticulturae E ff ects of bicarbonate induced iron chlorosis on photosynthesis apparatus in grapevine. Sci Hortic (Amsterdam) [Internet]. 2020;270(December 2019):109427. Available from: https://doi.org/10.1016/j.scienta.2020.109427
Kalaji HM, Jajoo A, Oukarroum A, Brestic M. Chlorophyll a fluorescence as a tool to monitor physiological status of plants under abiotic stress conditions. Acta Physiol Plant. 2016;38(102):1–11.
Collins EJ, Bowyer C, Tsouza A, Chopra M. Tomatoes: An Extensive Review of the Associated Health Impacts of Tomatoes and Factors That Can Affect Their Cultivation. Biology (Basel). 2022;11(2).
Jafarinia M, Shariati M. Effects of salt stress on photosystem II of canola plant ( Barassica napus , L .) probing by chlorophyll a fluorescence measurements. Iran J Sci Technol. 2012; 71–6.
Das HK. Azotobacters as biofertilizer [Internet]. 1st ed. Vol. 108, Advances in Applied Microbiology. Elsevier Inc.; 2019. 1-43 p. Available from: http://dx.doi.org/10.1016/bs.aambs.2019.07.001
Sumbul A, Ansari RA, Rizvi R, Mahmood I. Azotobacter: A potential bio-fertilizer for soil and plant health management. Saudi J Biol Sci [Internet]. 2020;27(12):3634–40. Available from: https://doi.org/10.1016/j.sjbs.2020.08.004
Raffi MM, Charyulu PBBN. Azospirillum-biofertilizer for sustainable cereal crop production: Current status [Internet]. Recent Developments in Applied Microbiology and Biochemistry. Elsevier Inc.; 2021. 193-209 p. Available from: http://dx.doi.org/10.1016/B9 78-0-12-821406-0.00018-7
Suhameena B, Devi S, Gowri R, Kumar A. Utilization of Azospirillum as a Biofertilizer – An Overview. Int J Pharm Rev Res. 2020;62(22):141–5.
Kalaji HM, Oukarroum A, Alexandrov V, Kouzmanova M, Brestic M, Zivcak M, et al. Identification of nutrient deficiency in maize and tomato plants by in vivo chlorophyll a fluorescence measurements. Plant Physiol Biochem [Internet]. 2014 Aug [cited 2014 Aug 31];81(April):16–25. Available from: http://www.ncbi.nlm.nih.gov/pubmed/24811616
Chen W, Jia B, Chen J, Feng Y, Li Y, Chen M, et al. Effects of Different Planting Densities on Photosynthesis in Maize Determined via Prompt Fluorescence, Delayed Fluorescence and P700 Signals. Plants. 2021;10(276):1–17.
Rosa WS, Martins JPR, Rodrigues ES, de Almeida Rodrigues LC, Gontijo ABPL, Falqueto AR. Photosynthetic apparatus performance in function of the cytokinins used during the in vitro multiplication of Aechmea blanchetiana (Bromeliaceae). Plant Cell Tissue Organ Cult [Internet]. 2018;133(3):339–50. Available from: http://dx.doi.org/10.1007/s11240-018-1385-x
Zubek S, Turnau K, Tsimilli-Michael M, Strasser RJ. Response of endangered plant species to inoculation with arbuscular mycorrhizal fungi and soil bacteria. Mycorrhiza [Internet]. 2009 Feb [cited 2014 Sep 1];19(2):113–23. Available from: http://www.ncbi.nlm.nih.gov/pubmed/19011910
Khalilpoor M, Jafarinia M. Investigation the Effects of Salinity and Nitric Oxide on the Changes of Chlorophyll a Fluorescence in Oat ( Avena sativa L .) Plant Probed by JIP-Test. Iran J plant biol. 2017; 9(31): 87-98.
Motamed S, Jafarinia M, Kholdebarin B. Investigating the effects of drought stress on photosynthetic electron transport chain of two basil ( Ocimum basilicum L .) cultivars by m easuring “ Chlorophyll - a ” fluorescence. J Biodivers Environ Sci. 2015;7(1):564–71.
Gupta R. The oxygen-evolving complex: a super catalyst for life on earth, in response to abiotic stresses. Plant Signal Behav [Internet]. 2020;15(12). Available from: https://doi.org/10.1080/15592324.2020.1824721
Paul S, Neese F, Pantazis DA. Structural models of the biological oxygen-evolving complex: Achievements, insights, and challenges for biomimicry. Green Chem. 2017;19(10):2309–25.
Cham R, Ali S, Jafarinia M, Yasrebi J. South African Journal of Botany Physiological responses of Dracocephalum kotschyi Boiss . to drought stress and Bio-fertilizers. South African J Bot [Internet]. 2022;148:180–9. Available from: https://doi.org/10.1016/j.sajb.2022.04.008
Vitale L, Vitale E, Guercia G, Turano M, Arena C. Effects of different light quality and biofertilizers on structural and physiological traits of Spinach plants. Photosynthetica [Internet]. 2020;58(4):932–43. Available from: https://doi.org/10.32615/ps.2020.039
Dimitrova S, Paunov M, Pavlova B, Dankov K, Kouzmanova M, Velikova V, et al. Photosynthetic efficiency of two platanus orientalis l. Ecotypes exposed to moderately high temperature – jip-test analysis. Photosynthetica [Internet]. 2020;58(Special Issue):657–70. Available from: https://doi.org/10.32615/ps.2020.012
Basset GJ, Latimer S, Fatihi A, Soubeyrand E, Block A. Phylloquinone (Vitamin K1): Occurrence, Biosynthesis and Functions. Mini-Reviews Med Chem. 2017;17(12).
Corpas FJ, González-Gordo S, Palma JM. Nitric oxide and hydrogen sulfide modulate the NADPH-generating enzymatic system in higher plants. J Exp Bot. 2021;72(3):830–47.
