Pyrene biodegradation by halo-tolerant yeast Basidioascus persicus

Subject Areas : Microbial Biotechnology

Aliyeh Kamyabi ¹ , Hamid Moghimi ²

1 - M.Sc. student, Department of Microbial Biotechnology, School of Biology, College of Science, University of Tehran, Tehran, Iran
2 - Assistant Professor, Department of Microbial Biotechnology, School of Biology, College of Science, University of Tehran, Tehran, Iran

Received: 2018-01-07 Accepted : 2018-03-19 Published : 2019-02-20

Keywords: Pyrene, Biodegradation, Basidioascus persicus, Saline conditions,

Abstract :

Background & Objectives: Poly-aromatic hydrocarbons are one of the most important toxic compounds of crude oil and environmental pollutants. The purpose of this study was to isolate halo-tolerant pyrene- degrading yeast.   Materials & Methods: Isolation of yeasts from contaminated and saline soils of oil-rich southern regions was carried out, at first. Then, screening of yeast isolates was performed based on their ability to grow in the salty medium and biodegrade oil. After screening and molecular identification of the optimal strain, its ability to tolerate salt and grow in the presence of pyrene, as well as the ability to degrade pyrene and other low molecular weight hydrocarbons were studied.   Results: In this study, EBL-C16 was selected as the superior isolate, with growth in salt concentrations of 0-15% and eliminating 75.51% of the crude oil. Molecular identification of this isolate showed 100% similarity to Basidioascus persicus. Growth analysis showed the ability of this yeast isolate to grow in salt concentrations of 0 to 20 %. Growth and removal studies in the presence of 500 mg/l of pyrene and 2.5% salt showed 78.57% pyrene removal after 21 days, with the growth rate of 1.4 g/l of dry weight, as well as CO2 production of 3.1 mg. B. persicus had the ability to break down phenanthrene and anthracene, as well.   Conclusion: The results can be used to use halo-tolerant yeasts for bioremediation of oil-contaminated saline areas.

References:

1. Yao L, Teng Y, Luo Y, Christie P, Ma W, Liu F, Wu Y, Luo Y, Li Z. Biodegradation of
   polycyclic aromatic hydrocarbons (PAHs) by Trichoderma reesei FS10-C and effect of
   bioaugmentation on an aged PAH-contaminated soil. Bioremediat J. 2015; 19(1): 9-17.
   2. Bamforth SM, Singleton I. Bioremediation of polycyclic aromatic hydrocarbons: current
   knowledge and future directions. J Chem Technol Biotechnol. 2005; 80(7): 723-736.
   3. Ortega-González DK, Cristiani-Urbina E, Flores-Ortíz CM, Cruz-Maya JA, Cancino-Díaz JC,
   Jan-Roblero J. Evaluation of the removal of pyrene and fluoranthene by Ochrobactrum
   anthropi, Fusarium sp. & their coculture. Appl Biochem Biotechnol. 2015; 175(2): 1123-1138.
   4. Zhou H, Wang H, Huang Y, Fang T. Characterization of pyrene degradation by halophilic
   Thalassospira sp. strain TSL5-1 isolated from the coastal soil of Yellow Sea, China. Int
   Biodeterior Biodegrad. 2016; 107: 62-69.
   5. Kamyabi A, Nouri H, Moghimi H. Synergistic effect of Sarocladium sp. and Cryptococcus sp.
   co-culture on crude oil biodegradation and biosurfactant production. Appl Biochem
   Biotechnol. 2017; 182(1): 324-34.
   6. Hassanshahian M, Zeynalipour MS, Musa FH. Isolation and characterization of crude oil
   degrading bacteria from the Persian Gulf (Khorramshahr provenance). Mar Pollut Bull. 2014;
   82(1): 39-44.
   7. Moghimi H, Tabar RH, Hamedi J. Assessing the biodegradation of polycyclic aromatic
   hydrocarbons and laccase production by new fungus Trematophoma sp. UTMC 5003. World J
   Microbiol Biotechnol. 2017; 33(7): 136.
   8. Zafra G, Moreno-Montaño A, Absalón ÁE, Cortés-Espinosa DV. Degradation of polycyclic
   aromatic hydrocarbons in soil by a tolerant strain of Trichoderma asperellum. Environ Sci
   Pollut Res. 2014; 1: 1-9.
   9. Castillo-Carvajal LC, Sanz-Martín JL, Barragán-Huerta BE. Biodegradation of organic
   pollutants in saline wastewater by halophilic microorganisms: a review. Environ Sci Pollut
   Res. 2014; 21(16): 9578-9588.
   10. Fathepure BZ. Recent studies in microbial degradation of petroleum hydrocarbons in
   hypersaline environments. Front Microbiol. 2014; 5.
   11. Pointing S. Feasibility of bioremediation by white-rot fungi. Appl Microbiol Biotechnol.
   2001; 57(1): 20-33.
   12. Acevedo F, Pizzul L, del Pilar Castillo M, Cuevas R, Diez MC. Degradation of polycyclic
   aromatic hydrocarbons by the Chilean white-rot fungus Anthracophyllum discolor. J Hazard
   Mater. 2011; 185(1): 212-219.
   13. Cui Z, Xu G, Gao W, Li Q, Yang B, Yang G, Zheng L. Isolation and characterization of
   Cycloclasticus strains from Yellow Sea sediments and biodegradation of pyrene and
   fluoranthene by their syntrophic association with Marinobacter strains. Int Biodeterior
   Biodegrad. 2014; 91: 45-51.
   
   14. Hui W, Haiyan Z, Yong HUANG TF. Isolation and degradation characteristics of a HMW
   -PAHs degrading halophilic strain. Tsinghua Sci Technol. 2015; 55(1): 87-92.
   15. Gomes M, Gonzales-Limache E, Sousa S, Dellagnezze B, Sartoratto A, Silva L, Gieg L,
   Valoni E, Souza R, Torres A. Exploring the potential of halophilic bacteria from oil terminal
   environments for biosurfactant production and hydrocarbon degradation under high-salinity
   conditions. Int Biodeterior Biodegrad. 2018; 126: 231-242.
   16. Hadibarata T, Khudhair AB, Kristanti RA, Kamyab H. Biodegradation of pyrene by
   Candida sp. S1 under high salinity conditions. Bioprocess Biosyst Eng. 2017; 40(9):
   1411-1418.
   17. u u h h u O’ v . L
   in fungal biology: current methods in fungal biology: Springer Science & Business Media;
   2012.
   18. Rahman K, Thahira-Rahman J, Lakshmanaperumalsamy P, Banat I. Towards efficient
   crude oil degradation by a mixed bacterial consortium. Bioresour Technol. 2002; 85(3):
   257-261.
   19. Heidarytabar R, Azin E, Moghimi H. Introduction of halotolerant Mucor circinelloides
   UTMC 5032 for bioremediation crude oil hydrocarbons. Biol J Microorganism. 2017; 6(21):
   31-45.
   20. Hesham AE-L, Wang Z, Zhang Y, Zhang J, Lv W, Yang M. Isolation and identification of
   a yeast strain capable of degrading four and five ring aromatic hydrocarbons. Ann Microbiol.
   2006; 56(2): 109.
   21. Sambrook J. Molecular cloning : a laboratory manual / Joseph Sambrook, David W.
   Russell. Russell DW, Cold Spring Harbor L, editors. Cold spring harbor, N.Y: Cold Spring
   Harbor Laboratory; 2001.
   22. Amberg DC, Burke DJ, Strathern JN. Methods in Yeast Genetics: A cold spring harbor
   laboratory course manual, 2005 Edition (Cold Spring). 2005.
   23. Balachandran C, Duraipandiyan V, Balakrishna K, Ignacimuthu S. Petroleum and
   polycyclic aromatic hydrocarbons (PAHs) degradation and naphthalene metabolism in
   Streptomyces sp.(ERI-CPDA-1) isolated from oil contaminated soil. Bioresour Technol.
   2012; 112: 83-90.
   24. Cerqueira VS, Hollenbach EB, Maboni F, Vainstein MH, Camargo FA, Maria do Carmo
   RP, Bento FM. Biodegradation potential of oily sludge by pure and mixed bacterial cultures.
   Bioresour Technol. 2011; 102(23): 11003-11010.
   25. Juckpech K, Pinyakong O, Rerngsamran P. Degradation of polycyclic aromatic
   hydrocarbons by newly isolated Curvularia sp. F18, Lentinus sp. S5, and Phanerochaete sp.
   T20. Sci Asia. 2012; 38: 147.
   26. Singh H. Mycoremediation: fungal bioremediation: John Wiley & Sons; 2006.
   
   27. Cerniglia CE. Fungal metabolism of polycyclic aromatic hydrocarbons: past, present and
   future applications in bioremediation. J Ind Microbiol Biotechnol. 1997; 19(5): 324-333.
   28. Pozdnyakova NN, Rodakiewicz-Nowak J, Turkovskaya OV, Haber J. Oxidative
   degradation of polyaromatic hydrocarbons catalyzed by blue laccase from Pleurotus ostreatus
   D1 in the presence of synthetic mediators. Enzyme Microb Technol. 2006; 39(6): 1242-1249.
   29. Deng Y, Zhang Y, Hesham AE-L, Liu R, Yang M. Cell surface properties of five
   polycyclic aromatic compound-degrading yeast strains. Appl Microbiol Biotechnol. 2010; 86
   (6): 1933-1939.
   30. Chen B, Wang Y, Hu D. Biosorption and biodegradation of polycyclic aromatic
   hydrocarbons in aqueous solutions by a consortium of white-rot fungi. J Hazard Mater. 2010;
   179(1): 845-851.
   31. Arun A, Eyini M. Comparative studies on lignin and polycyclic aromatic hydrocarbons
   degradation by basidiomycetes fungi. Bioresour Technol. 2011; 102(17): 8063-8070.
   32. Ting W, Yuan S, Wu S, Chang B. Biodegradation of phenanthrene and pyrene by
   Ganoderma lucidum. Int Biodeterior Biodegrad. 2011; 65(1): 238-242.
   33. Ghosh I, Mukherji S. Diverse effect of surfactants on pyrene biodegradation by a
   Pseudomonas strain utilizing pyrene by cell surface hydrophobicity induction. Int Biodeterior
   Biodegrad. 2016; 108: 67-75