Frequency of meticillin resistant Staphylococcus aureus strains isolated from clinical samples in Mousavi Hospital, Zanjan, and recognition mec A gene using PCR

Subject Areas : Medical Microbiology

Rasoul  Shokri ¹ (MS.c., Department of Microbiology, Siences and Research Branch, Islamic Azad University, Fars, Iran)
Mojtaba  Salouti ² (Associate Professor, Biology Research Center, Zanjan Branch, Islamic Azad University Zanjan, Iran.)
Rahim  Sorouri Zanjani ³ (Associate Professor, Department of Microbiology, Zanjan University of Medical Siences, Zanjan, Iran.)
Zahra  Heidari ⁴ (MS.c., Biology Research Center, Zanjan Branch, Islamic Azad University Zanjan, Iran.)

Received: 2013-12-10 Accepted : 2014-02-17 Published : 2014-04-01

Keywords: Staphylococcus aureus, PCR, Antibiotic Resistance, mecA gene,

Abstract :

Background and Objectives: Nowadays Meticillin resistant Staphylococcus aureus (MRSA) is one of the public-health threats due to resistance to agents and anti microbial drugs. The aim of present study was to find the incidence of Meticillin resistant Staphylococcus aureus strains isolated from clinical samples in Ayatollah Mousavi Hospital of Zanjan and their antibiotic resistance pattern as well as recognizing of the mecA gene using PCR. Materials  &Methods: In this descriptive cross-sectional study, 176 specimens were collected from different sections of Ayatollah Mousavi Hospital and assayed. The strains were identified and the resistances of the isolates to 12 kinds of antibiotics were determined using disk diffusion method. Finally, following DNA extraction, mecA gene was analyzed by PCR. Results: 45 Staphylococcus aureus isolates were recovered (25.56%). 26 out of 45 Staphylococcus aureus isolates (57.77%) were confirmed as MRSA. Evaluation of antibiotic resistance showed the greatest resistance to penicillin (100%) and cloxacillin (80.76%), respectively, and the lowest resistance was observed to vancomicin (7.69%). Conclusion: The findings showed that the prevalence of MRSA was remarkable in the hospital samples and the resistance to methicillin has increased that is a serious warning to the treatment of infections caused by this bacterium.