Evaluation of pathogenicity and polymorphism of ompH gene in Pasteurella multocida

Subject Areas : Molecular Microbiology

Maryam Oulad ¹ , Yahya Tahamtan ² , Nooshin Sohrabi ³

1 - Master Genetic Graduated, Payam Noor University, Faculty Tehran-Center, Rey, Tehran, Iran.
2 - Associated Professor, Shiraz branch, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Shiraz, Iran.
3 - Assistant Professor, Department of Biology, Payam Noor University, Faculty Tehran-Center Pardis new town, Tehran, Iran.

Received: 2017-04-02 Accepted : 2017-05-26 Published : 2018-02-20

Keywords: Polymorphism, Pasteurella multocida, RFLP-PCR,

Abstract :

Background & Objectives: Pasteurella multocida is a Gram-negative facultative bacterium, causing respiratory diseases in various domestic and wild animals. The aim of this study was isolation and identification of P. multocida and investigation of ompH gene polymorphism of in goats P. multocida isolates. Material & Methods: In a cross-sectional study a total of 160 swab samples from nose and throat of goats infected with Pasteurellosis in Fars province were collected. The ompH gene polymorphism in 26 isolates was genotyped by PCR-RFLP technique. Restriction digestion was performed using HindIII and EcoRI endonuclease enzymes. Each sample was injected into two mice at a concentration of 0.5 McFarland's standard. After ompH gene digestion by endonuclease enzymes, RFLP patterns were compared with mice mean dead time (MDT). Results: Each HindIII and EcoRI enzymes made different patterns, which were remarkable in comparison with MDT in mice. All lethal isolates had MDT less than 24 hours. Out of 29 P. multocida isolates,  89.6 % were lethal in mice. Conclusion: According to established patterns, MDT in mice was different. Based on the results, the samples with more frequent patterns killed mice in a shorter time and were more lethal. Enzyme digestion patterns can be used to detect virulent and pathogenic strains and to provide vaccine strains. Considering the variability of these strains, the production of multiple vaccines is necessary.