Study on isolated staphylophage from waste water on detected Staphylococcus from raw milk
Subject Areas : Food Microbial Contaminationنیما بهادر 1 * , Maryam Hossein sarbas 2 , Bahar Ajam Lotfi 3 , Maedeh Noshadi 4 , Mahmonir Bahramian 5 , Seydeh Golnaz Khani 6
1 - دانشگاه آزاد شیراز
2 - گروه میکروبیولوژی، دانشگاه ازاد اسلامی واحدشیراز
3 - Department of Biology, Zand Institute
4 - Department of Biology, Zand Institute of Higher education, Shiraz
5 - Department of Food Science and Industry,
6 - Department of Biology, Zand Institute of Higher education
Keywords: Staphylococcus aureus, mastitis, milk, staphylophage,
Abstract :
Staphylococcus aureus is an important agent of mastitis among animal with capability to produce dairy compounds. Nowadays, the organism is recognized as multi drug resistant organism. Therefore, the aim of this study is isolation of Staphylococcus aureus from raw milk of cows infected with mastitis and the evaluation of bacteriophage on the same organisms. In this study 50 milk samples were collected from suspected cows with mastitis. The samples were cultivated on mannitol salt agar and the supposed colonies were identified using gram stain and biochemical tests. Then antibiotic pattern were evaluated. On the other hand, the sewage samples were collected and based on some tests including: electron micrograph, nucleic acid type, host range, pH bacteriophages were identified. Results indicated that out of 50 samples 6 isolates were confirmed using biochemical tests. In addition among antibiotics, chloramphenicol, ciprofloxacin, and gentamycin 100% had effect on the isolates and the other antibiotics showed different results. Detected phages showed clear plaque at room temperature and 37C, and they were belong to siphoviridae family with DNA, and had effect on gram positive bacteria at neutral pH. The results illustrated that phages had effect on Staphylococcus aureus and we could use them as biocontrol agents against infections.
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