Investigating the antimicrobial effects of lemongrass and lavender extracts and essential oils with antibiotics on some food bacteria
Subject Areas : Industrial Food Microbiology
Mehrdad Ataie Kachouei
1
,
Elham Fakhri
2
,
fatemeh khodaverdipour
3
1 - Department of Medicinal Plants, College of Food and Drug, Islamic Azad University, Shahrekord
Branch, Shahrekord, Iran.
2 - M.Sc. Graduate of Physiology and improvement of medicinal, spice and aromatic plants, Faculty of agriculture, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
3 - M.Sc. Graduate of Microbiology, Faculty of basic sciences, Shahrekord Branch, Islamic Azad University,
Shahrekord, Iran
Keywords: Lemon balm, lavender, extract, , essential oil, antibacterial effects,
Abstract :
In recent years, the increase in drug resistance against bacteria and the increased dosage of common drugs and antibiotics, as well as the side effects caused by these drugs, have brought natural factors such as medicinal plants with fewer side effects, which have received more attention. increase. For this reason, in this study, we prepared alcoholic extracts and essential oils from lemongrass and lavender plants and then serially diluted them to obtain the minimum growth inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against Shigella Flexnerl, Salmonella typhimuriom, Esherichia coli and Staphylococcus aureus bacteria were prepared. Zones of inhibition of bacterial growth were also measured and compared using the disc diffusion method. The results obtained showed that the halo diameter of the essential oil was larger than that of the extract, indicating a higher susceptibility of the sample to the Gram-positive bacterium Staphylococcus aureus. The amount of MIC and MBC in essential oil and extract, respectively, against each of the four bacteria tested was measured by microdilution method, which showed that the concentration of MIC and MBC in essential oil was lower than in extract. Analysis of compounds in each essential oil and extract was performed using a GC-MS device, essential oils contain more compounds than extracts and therefore have more antibiotic properties. Therefore, the results of this study demonstrate that lemongrass and lavender essences and extracts have antibacterial effects. Therefore, they can be used as natural plant products to combat bacterial infections.