• Home
  • Study on the Frequency of Vibrio species in fish from Isfahan

Share To

Article Url


Manuscript ID : JFM-2010-1678 (R3) Visit : 167 Page: 47 - 55

10.30495/jfm.2021.683634

Article Type: Original Research

Study on the Frequency of Vibrio species in fish from Isfahan

Subject Areas : Food Microbial Contamination

Abbas Karimi Alavigeh 1 ( Graduate of Pharmacy Shahreza Branch, Islamic Azad University, Shahreza, Iran )
Ali Sharifzadeh 2 ( IAU, Shahrekord )

Received: 2020-10-21 Accepted : 2021-01-19 Published : 2021-12-22

Keywords: Contamination, fish, Vibrio, Key Words: PCR,

Abstract :

Annually, many reports of occurrence of food poisoning due to the consumption of sea-foods contaminated with Vibrio species have been reported. The present survey was carried out to evalution the frequency rate of Vibrio species in sea-food products from the fish samples in Isfahan, Iran.In this survey, 64 fresh fish samples were purchased from markets in Isfahan and transferred to the food quality control laboratory of the Islamic Azad University of Shahreza . Samples were tested for count Vibrio parahaemolyticus and detection according to Iranian national standard methods . positive specimens were evaluated for presence of bacterial species using Nested Polymerase Chain Reaction . the presences of Vibrio parahaemolyticus, Vibrio cholera, Vibrio vulnificus and Vibrio mimicus were studied using the Nested PCR method. Results showed that 95% of samples were contaminated with Vibrio species. In studied samples, V. parahaemolyticus had the highest frequency rate (35%), while V. mimicus had the lowest frequency rate (15%). Frequency of V. cholera and V. vulnificus were 20% and 35% respectively. The results of microbial investigations of raw fish collected from different markets of Isfahan can not be defined satisfactorily . High prevalence of Vibrio species in samples confirmed the lack of hygienic condition in the production and distribution centers of fish . It seems that, places of fishing and processing and the moods of transportation and distribution of fish don’t have suitable hygiene in Isfahan .

References:

References

  1. سازمان ملی استاندارد ایران . (1378) استاندارد شماره 2394. چاپ اول.
    2.
  2. حسینی سحر، صفرپوردهکردی فرهاد، رحیمی ابراهیم، شاکریان امیر. (1393). مطالعه میزان شیوع گونه های ویبریو و فراوانی ژنهای حدت در ویبریو پاراهمولیتیکوس های جداسازی شده از میگوهای تازه و نمک سود شده در بندرگناوه. جلد4، شماره2، صفحه 27-17 .
    3.
  3. جلالی محمد، مهدوی منیژه، جوادی عباسعلی، خوروش فرزین، عطایی بهروز، عابدی داریوش .(1388). آلودگی ویبریو پاراهمولیتیکوس در اغذیه دریایی شهر اصفهان. مجله بیماریهای عفونی و گرمسیری ایران. جلد14، شماره46 ، صفحه37-33.
    4.
  4. Ansari, M. and Raissy, M. In vitro susceptibility of commonly used antibiotics against Vibrio spp. isolated from Lobster (Panulirus homarus). Afr J. Microbiol Res. 4(23), 2629-2631.
    5.
  5. Elmahdi S, DaSilva LV, Parveen S. Antibiotic resistance of Vibrio parahaemolyticus and Vibrio vulnificus in various countries: A review. Food Microbiol. 57, 128-34.
    6.
  6. Hang L. 2012. Community compositions of Vibrio in freshwater products and pathogenic analysis. Dissertation for Master degree, Yangzhou: Yangzhou University, 19–35.
    7.
  7. Heng SP, letschumanan V, deng CU, Abmutalib NS, Khan TM, Chuah LH, Chan K.G., Goh B.H, Pusparajah P., Lee L.H. 2017. Vibrio vulnificus: An Environmental and Clinical Burden. Front Microbiol. 8(997): 73-81.
    8.
  8. Jaksic S, Uhitil S, Petrak T, Bazuli D, KarolyiLG. 2002. Occurrence of Vibrio spp. in sea fish, shrimps and bivalve molluscs from Adriatic Sea. Food Cont. 13, 491-493.
    9.
  9. Lee Y, Choi Y, Lee S H, Lee H, Kim S, Ha, J. 2019. Occurrence of pathogenic Vibrio parahaemolyticus in seafood distribution channels and their antibiotic resistance profiles in South Korea. Let Appl Microbiol. 68(2), 128-133.
    10.
  10. 1Lopatek M, Wieczorek K, Osek J. 2018. Antimicrobial Resistance, Virulence Factors, and Genetic Profiles of Vibrio parahaemolyticus from Seafood. Appl Environ Microbiol. 84(16), 1-10
    11.
  11. MarkeyB,LeonardF,ArchambaultM,CullinaneA,MaguireD.2013.Clinical Veterinary micro
    12.
  12. Shirazi MH, Ranjbar R, Salari MH, Bagheri Y, Najafi A and Sadeghifard N. 2007. Isolation of Vibrio parahaemolyticus from fish in Tehran and their antimicrobial resistance. Iran J Infect Diseases Trop Med. 11(35), 65-68.
    13.
  13. Srivastava A, Singh AN, Singh M. 2016. Antibacterial activity of spices against Vibrio species isolated from pond water. European J Exp Biol. 6(2), 21–25.
    14.
  14. Su YC, Liu C. 2007. Vibrio parahaemolyticus: A concern of seafood safety. Food Microbiol. 24(6),549- 558
    15.
  15. Talukder B, Chakma F, Sabuj AAM, Haque ZF, Rahman MT, Saha S.2019. Identification, prevalence and antimicrobial susceptibility of major pathogenic bacteria in exportable shrimp (Penaeus monodon) of southern Bangladesh. Int J Fish Aquat Stud. 7(1), 9669.
    16.
  16. Tarrc L, Pate JS, Puhr ND, Sowers EG, Bopp CA, Strockbine NA.2007. Identification of Vibrio Isolates by a Multiplex PCR Assay and rpoB Sequence Determination, J Clinic Microbiol. 45(1), 134-140.
    17.
  17. Thompson CC, Thompson FL,Vicente AC. 2008.Identification of Vibrio cholerae and Vibrio mimicus by multilocus sequence analysis (MLSA). Int J Syst Evol Microbiol. 58(3), 617-621.
    18.
  18. Tille P.2014. Bailey & Scott's Diagnostic Microbiology, 14th edition. Elsevier. pages: 175-202.
    19.
  19. Maheshwari M, Krishnaiah N, Ramana DBV.2011. Evaluation of Polymerase Chain Reaction for the detection of Vibrio cholerae in Contaminants. Ann Biol Res. 2(4), 212-217.
    20.
  20. Mohammed Y, Aboderin AO, Okeke IN, Olayinka AT. 2018. Antimicrobial resistance of Vibrio cholera from sub-Saharan Africa: a systematic review. Afr J Lab Med. 7(2), 1–7.
    21.
  21. Obaidat MM, Salman AEB, Roess AA. Virulence and Antibiotic Resistance of Vibrio parahaemolyticus Isolates from Seafood from three developing countries and of worldwide environment seefood and clinical isolates from2000 to 2017 .80(12), 2060-2067
    22.
  22. Raissy M, Moumeni M, Ansari M and Rahimi E. 2012. Antibiotic resistance pattern of some Vibrio strains isolated from seafood, Iran J Fish Sci. 11(3), 618-626.
    23.
  23. Raissy, M. Moumeni, M. Ansari, M and Rahimi, E. 2012. Occurrence of Vibrio in Lobster and Crab from the Persian Gulf, J Food Saf. 32(2), 198-203. https://doi.org/10.1111/j.1745-4565.2012.00368.x
    24.
  24. Ruichao L, Chiou J, Chan EW, Chen S. A 2016. Novel PCR-Based Approach for Accurate Identification of Vibrio parahaemolyticus, Front Microbiol. 7(44), 23-29.
    25.
  25. Safarpoor Dehkordi F, Hosseini S , Rahimi E , Momeni M , Yahaghi E , Khodaverdi E.2014. Study the distribution of virulence genes of Vibrio parahaemolyticus isolated from fish, lobster and crab caught from Persian Gulf. Iran J Med Microbiol. 8(2),1-7.
    26.
  26. Scallan, E., Hoekstra, R.M., Angulo, F.J., Tauxe, R.V., Widdowson, M.A., Roy, S.L., et al. 2011. Foodborne illness acquired in the United States-major pathogens. Emerg Infect Dis, 17(1), 7-15.
    27.
  27. Senachai P, Chomvarin C, Namwat W, Wongboot W, Wongwajana S, Tangkanakul W. 2013. Application of tetraplex PCR for detection of Vibrio cholerae, V. parahaemolyticus, V. vulnificus and V. mimicus in cockle. Southeast Asian J Trop Med Publ Health. 44(2), 249- 58.
    28.
  28. Wey S, Zhao H, XianY, Ahossain M, Wu X. 2014. Multiplex PCR assays for the detection of Vibrio alginolyticus, Vibrio parahaemolyticus, Vibrio vulnificus, and Vibrio cholerae with an internal amplification control, Diag Microbiol Infect Dis.79, 115-118.
    29.
  29. Zereen F, Akter S, Abdus Sobur M, Hossain MT, Rahman T. Molecular detection of Vibrio cholera from human stool collected from SK Hospital, Mymensingh, and their antibiogram. J Adv Vet Anim Res. 6(4), 451-455
    30.
_||_

Related articles

The rights to this website are owned by the Raimag Press Management System.
Copyright © 2021-2024

Home| Login| About Us| Contact Us|
[فارسی] [العربية] [fa] [ar]