Investigation of Influencing Factors on Production of Chlorophyll and Carotenoid Pigments from Spirulina Platensis Using Platelet-Burman Design

Subject Areas : Industrial Food Microbiology

Sepideh Banayan ¹ , Mahshid Jahadi ² , Mohammad Fazel ³

1 - Department of Food Science and Technology, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
2 - Department of Food Science and Technology, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran.
3 - Department of Food Science and Technology, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran.

Received: 2019-08-28 Accepted : 2019-11-27 Published : 2020-06-21

Keywords: Chlorophyll, Carotenoid, pigment, Spirulina platensis, Placket-Burman design,

Abstract :

Spirulina platensis has attracted particular attention because of its importance as food, feed and natural pigments with specific functional properties .The purpose of this study is determination and screening of the most important factors including: light-dark cycles (12h light/12h dark and 16h light/8h dark), cultivation period (11 and 14 days), light-emitting diodes (LED) composition (6 blue/ 3 red/ 4 white  and 3 blue/6 red/4 white), nitrogen source (65 mg/l urea in fed batch mode and 40 mg/l urea in batch mode), carbon source (1 g/l date syrup in batch and fed batch mode),  inoculation size (OD: 0.4 and 0.6) and sodium chloride content (0 and 2 g/l) on the chlorophyll a and carotenoids pigments production in Spirulina Platensis using the Placket-Burman design. The results showed that among the investigated factors, sodium chloride, carbon source, light-emitting diodes composition, Cultivation period (p<0.01) and inoculation size (p<0.05) had significant effect on chlorophyll-a production and nitrogen source, carbon source and sodium chloride had significant effect (p <0.01) on carotenoids production. The highest production of these pigments was obtained 13.46 ± 0.9 and 8.21± 0.17 (μg/ml) for chlorophyll-a and carotenoids, respectively.