Antimicrobial effects of Chitosan films incorporating with Lavandula stoechas on some food-borne bacteria

Subject Areas : Food Microbial Contamination

Zohreh Mashak ¹ , سمیرا فیاض فر ² , نرجس چراغی ³

1 - Department of Food Hygiene, College of Veterinary Medicine, Islamic Azad University of Karaj, Karaj, Iran.
2 - Studenet of Veterinary Medicine, College of Veterinary Medicine, University of Tehran, Tehran, Iran.
3 - ph.D Student of Food Hygiene and Quality Control, College of Veterinary Medicine, University of Tehran, Tehran, Iran.

Received: 2015-10-03 Accepted : 2016-05-04 Published : 2016-05-01

Keywords: Chitosan, Foodborne pathogenic bacteria, Lavandula stoechas essential oil,

Abstract :

Today, application of natural antimicrobial films as an inhibitors of growth of dangerous microbes especially in food staffs have been increased. The present study was carried out in order to study the antimicrobial activity of chitosan films containing 1–4% (v ⁄ v) Lavandula stoechas essential oil (EO) on some food-borne pathogenic bacteria (Listeria monocytogenes, Bacillus cereus, Vibrio parahaemolyticus, Staphylococcus aureus and Escherichia coli O157:H7). At first, the essential oil of the Lavandula stoechas herb was prepared. The most essential components are 1,8 cineol (16/49%), borneol (75/10%), camphor (94/8%) and linalool L (95/3%) and alfa-pinene (2/3 %).Then Circular discs of chitosan films containing different concentration of Lavandula stoechas essential oil were placed on bacterial lawn. Chitosan films incorporated with 4% (v ⁄ v) of EO inhibited the growth of all tested pathogenic bacteria. Incorporation of Chitosan film with EO showed significant antimicrobial effects (P < 0.05). Transparency of film was reduced by increasing of EO. These results showed that Chitosan films containing Lavandula stoechas EO have good effect for packaging some foods.