Determining the presence of virulence genes Panton Valentine leukocidin PVL and methicillin gene mecA in Staphylococcus aureus

Subject Areas : Food Microbial Contamination

کیومرث امینی ¹ , سجاد علیزاده ²

1 - استادیار/ دانشگاه آزاد اسلامی واحد ساوه
2 - دانشجو/دانشگاه آزاد اسلامی سیرجان

Received: 2015-05-05 Accepted : 2015-06-16 Published : 2015-06-01

Keywords: Staphylococcus aureus, Methicillin, Pantone Valentine leukocidin, Multiplex- PCR,

Abstract :

Staphylococcus aureus is a major cause of food poisoning in the world that is created by consumption of contaminated food. Resistance to a variety of common and specific antibiotics is increasing. Staphylococcus aureus including PVL and gene mecAto heat pasteurization and many proteolytic enzymes are stable and can remain active for a long time in food samples. The purpose of this study was to isolate Staphylococcus aureus and identify virulence gene PVL and gene of methicillin resistance in food samples by multiplex PCR technique has been used. The study included 120 samples of various foods (dairy, confectionery, meat and vegetables) collected 40 cases (33/3%) Staphylococcus aureus isolates were detected. Antibiotic susceptibility testing by disk diffusion method according to CLSI guidelines was conducted. To identify and confirm Staphylococcus aureus virulence and resistance genes from multiple PCR assay was used. Antibiogram results showed that antibiotics are among the most sensitive to the antibiotic vancomycin, Teicoplanin and methicillin respectively 95%, 90% and 75%. Resistance to linezolid, azithromycin and methicillin respectively, 35%, 32% and 25% more than other antibiotics was tested. Prevalence of methicillin resistance gene mecAin total 57.5% and PVL gene was not detected. Also 16srRNA gene in all samples was identified genus and species and confirmed. Different distribution of methicillin resistance gene in this study with other studies showing the potential risk of methicillin resistant Staphylococcus aureus in the world. Therefore, early detection and timely resistant strains, in order to prevent the spread of resistance appears to be necessary.