Detection of Vibrio parahaemolyticus in shrimp samples collected from Ardabil by PCR method

Subject Areas : Food Microbial Contamination

Daryoush Asgarpoor ¹ , Mehdi Ghasemi ² , Mahrokh Bahrami ³

1 - Department of Microbiology and Virology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
2 - Department of Microbiology, Ardabil Branch, Islamic Azad University, Ardabil, Iran
3 - Department of Microbiology, Ardabil Branch, Islamic Azad University, Ardabil, Iran

Received: 2017-08-12 Accepted : 2016-10-12 Published : 2018-10-23

Keywords: PCR, Shrimp, Vibrio. parahaemolyticus,

Abstract :

Vibrio parahaemolyticus, a member of family Vibrionaceae, is a non-spore-forming, rod-shaped, gram-negative and halophilic bacterium with a single polarflagellum known as seafood-borne gastroenteritis throughout the world. These bacteria are characterized by three major clinical manifestations, including wound infection, primary septicemia and gastroenteritis. Accordingly, the present cross-sectional study aimed to detect V. parahaemolyticus using PCR method in 80 fresh shrimp samples collected from different seafood centers in Ardabil, Iran. The samples after immediately homogenization were cultured in TCBS agar. The resulting isolates were identified by biochemical tests, and then typical colonies were selected for DNA extraction. Next, the PCR technique was employed to detect the presence or absence of vp-toxR gene as a marker of these bacteria. Based on the findings, 11 (13.75 %) out of 80 fresh shrimp samples were positive for V. parahaemolyticus. According to the results, shrimp could be the major sources of V. parahaemolyticus thathave a potential risk to the public health inasmuch as might be viable and infectious.