The effect of letrozol as aromatase inhibitor drug on sex steroids in mature and immature female Cichlosoma nigrofasciatum

Subject Areas : Marine Biology

T. Naji ¹ , H. Hosseinzade Sahafi ² , M. K. Jazebizadeh ³ , M. Majidi ⁴

1 - Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran
2 - Iranian Fisheries Research Organization, Tehran, Iran
3 - Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran
4 - Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran

Received: 2010-09-21 Accepted : 2010-12-21 Published : 2011-06-20

Keywords: Aromatase inhibitors, Letrozole, Steroids, Zebrafish,

Abstract :

In this study, the effect of letrozole drug as  aromatase inhibitor on changes of some Sex steroids on mature and immature female Zebrafishwas investigated. For this Purpose, after dechlorination of aquarium water, mature fishes with mean weight (0.32 ±0.5 g) and immature (0.15±0.5 g) were released, experiments in 8 treatments (2 treatments as control and six treatments as experimental) were done. Different doses of Letrozole 10, 20 and 30 µg per kg fish into Intramuscular injection (IM)Dictionary - View detailed dictionary 1.noun 1.injection 2.infusion 3.transfusion 4.shot 5.intinction  and the amount of 0/001 ml was injected and the injections in 10 days( every other day) were performed. Then hormones( estradiol and testosterone) in experimental treatments were measured  The results of comparing the level of steroids among treatments of different showed  that  testestron amount increased  in mature fish (0.04 ،0.63، 0.62، 0.82 µg/kg )treated compared with control treatment and  in third treatment  increased much more than the other two were treated and also  estradiol amount  decreased (2.7 ،1.6، 1.85، 2.05 µg/kg )  in all treatments that represented the inhibitory function of Letrozole drug(p<0/05).