Evaluation of anti carcinogenic effect of Artemia urmiana by using method of Ames
Subject Areas : Marine Biology
S. Mehrabian
1
(Faculty of Science, Islamic Azad University, North Tehran Branch)
M. Abbasi
2
(Islamic Azad University, Malakan Branch)
Keywords: Antioxidant, Artemia urmiana, Ames test, Anticarcinogenicity,
Abstract :
Free radicals are produced during oxidative metabolism in body and they can attack to all of component of cells and cause cellular changes and early aging. Antioxidant materials role for balance maintenance in cells has currently attracted more attention. The aim of this study is investing of antimutation effect of Artemia, cyst and decapsulated egg extract by Ames test and Salmonella typhimurum TA 100 strain. These extract prevent reverse mutation caused by carcinogenic materials. In comparison with positive controls (sodium azid, KMno4, cresol) and negative control (distilled water) antimutagenicity properties of Artemia was evaluated and confirmed. In addition, the value of Astaxanthin in those extracts was assayed. for quality analysis and separation of Astaxanthin, we used TLC chromatography and column chromatography, respectively. Then, the presense of Astaxanthin in these extracts was confirmed by spectrometer IR test. The results showed that antimutagenicity properties of Artemia urmiana are the same with other salty water Artemia. Antimutagenicity and anticarcinogenicity properties in decapsulated egg, dried artemia and cyst are %85, %70 and %100 respectively.