Comparison of efficacy of 2-phenoxyethanol, clove oil and PI222 as anesthetics on the Silver carp (Hypophtalmichthys moltitrix)

Subject Areas : Marine Biology

S. Tojar ¹ , M. Khodadadi ² , M. Javaheri ³

1 - Fisheries Dept., Islamic Azad University, Science and Research Branch of Ahvaz
2 - Fisheries Dept., College of Agriculture and Natural Resources, Islamic Azad University, Ahvaz Branch, Iran
3 - Fisheries Dept., College of Agriculture and Natural Resources, Islamic Azad University, Ahvaz Branch, Iran

Received: 2011-08-10 Accepted : 2011-11-21 Published : 2011-12-21

Keywords: PI222, Anesthetic, Hypophtalmichthys Moltitrix, 2-Phenoxy ethanol, clove oil,

Abstract :

This study has considered the determination and comparison of the appropriate dosage of three anaesthetics including: 2-phenoxy ethanol, clove oil and PI222 on silver carp fish (Hypophthalmichthys molitrix). Based on reduction of anesthesia time in fish to less than 3 minutes and recovery to less than 5 minutes. So 120 fish were studied. We use 4 dose for each of anesthetics and 10  fish for each dose ; For 2-phenoxy ethanol ,doses of 250, 300, 350 and 400 part per million ,and for clove oil and PI222, doses of 25, 50, 75 and 100 part per million were considered. Indicating 300 ppm as the best dosage for 2-phenoxy ethanol, and 50 ppm as the best dosage for clove oil and PI222. In comparison of these substances, clove oil and PI222 have priority to 2-phenoxy ethanol; and this priority is more remarkable in PI222, because of being cheaper and safety for fish and user. Analysis variance of result indicates that the anesthesia induction time had a significant relationship with increasing dosage of the three anesthetics (P<0/05) and this period decreased by increasing the dosage. The time of recovery was not significant (P<0/05) and performed independent from the dosage.