Identification of fatty acids composition in fresh and frozen silver carp (Hypophthalmichthys molitrix) for determination of its shelf life at -18ْc
Subject Areas : Marine BiologyA. R. Najafi 1 , N. Beheshtiseresht 2
1 - Delvar afzar Company
2 - Razi Vaccine & Serum Research Institute
Keywords: freezing, Gas Chromatography, Hypophtalmichthys molitrix, Fridge, Fat extraction, Saturated fatty acids, unsaturated fatty acids,
Abstract :
In this work, after extraction and methylation of the lipids form silver carp tissues in fresh and frozen stages and their changes during 6 months in -18°C, identification of fatty acids of the samples were done by gas chromatography (Shymatsv HSSA4). Results of this investigation showed that amounts of unsaturated and saturated fatty acids in fresh samples were 70/95% and 28/95% respectively. In fresh tissues the amount of oleic acid was 30/16%, and Iino leice amount was 11/44%. Alpfa – lino lenice acide was 4/41%, Gcosapanethanoic was 6/42% and docosa hexanoic was 6/15%. These were the most important poly-saturated fatty acids. But after six months of freezing at -18° c the amounts of these fatty acids decreased to 10/02%, 3/18%, 2/97%, 1/05% and 1/02% respectively. Results showed a decrease in freezing condition of fatty acids. Based on these results, the best storage for frozen fish in -18°C is four months. These results were subjected to tests of Tukey and Analysis of Variance and were significant at the level of 95%.
افشار مازندران، ن. 1381. راهنمای علمی تغذیه نهاده های غذایی و دارویی آبزیان در ایران . چاپ اول. انتشارات نوربخش . تهران .
گلشاهی ، ع. 1381. شناسایی اسیدهای چرب تشکیل دهنده ماهی کیلکا و تغییرات آن حین فرآیند صنعتی تولید آرد ماهی.پایان نامه کارشناسی ارشد دانشگاه آزاد اسلامی واحد علوم و تحقیقات. تهران.
گلشاهی،ع. 1378 . اصول تغذیه ماهیان گرمابی. دانشگاه آزاد اسلامی واحد علوم و تحقیقات . تهران.
معینی ، س. 1368 . صنایع فرآورده های شیلاتی . سازمان تحقیقات و آموزش شیلات ایران ،تهران.
هدایتی فرد ، م. 1381 . شناسائی کمی و کیفی اسیدهای چرب بافت ماهی کفال طلائی (liza avrevta) مجله علوم دریائی ایران ، 2: 77 – 73 .
هدایتی فرد، م. و معینی ، س. 1381 . بررسی ترکیب اسیدهای چرب در ماهی ازون برون در شرایط تازه و منجمد . مجله علوم دارویی ایران، 6ص .
هدایتی فرد ، م. 1380. تکنولوژی انجماد فراورده های دریایی . انتشارات معاونت اطلاعات علمی ، مرکز تحقیقات شیلاتی مازندران ، ساری.
Aggelosis, G. & lazos, E.S. 1991 .Fatty acid composition of the lipid from eight fresh water fish species from Greece. Journal of Food Composition and Analysis, 4:68-76.
Eid, N., Dashti, B. & Sawaya,W. 1992 .Chemical and physical characterization of shrimp by- catch of the Arabian (persian) Gulf . Food Research International, 25:181-186.
Hall.G.M.(ed.).1997.Fish Processing Technology. 2th.ed.Blackie Academic& Professional, Chapman and Hall Press, USA.
Huss.H.H.1994 .Quality and quality changes in fresh fish. F.A.O .NO.348.
Meydani .S.N.1994 Interaction of w-3 polyunsaturated Fatty Acids and vitamin E on the Immune Response in: Fatty Acids and lipids. Galli, C., Simoppulos, A.P. & Tremoli, E. (Eds.). Vol 75. karger, New York.
Murph, R.G. 1993. Handbook of Lipid Research, 7, Mass Spectrometry of Lipids. Plenum Press, New York, USA.
Stansby . M.E.(ed).1990 . Fish oils in nutrition. AVI, Van Noustr and Reinhold, New York.