Effect of hydrogen peroxide based on silver colloid on decapsulation of Urmia Lake Artemia cysts (Artemia urmiana) to achieve the best hatching rate
Subject Areas : Marine BiologyR. Mosavi Nodoushan 1 , S. Farahnak 2
1 - Dept. of Fisheries, Faculty of Marine Science and Technology, Islamic Azad University, Tehran North Branch
2 - Dept. of Fisheries, Faculty of Marine Science and Technology, Islamic Azad University, Tehran North Branch
Keywords: Hatching rate, Artemia cysts, decapsulation, hydrogen peroxide based on silver colloid,
Abstract :
This study aimed to determine the most appropriate dose of hydrogen peroxide based on silver colloid for the decapsulation of Urmia Lake Artemia cysts (Artemia urmiana), to achieve the best hatching rate. The cysts were exposed to doses of 0.5, 0.75, 1, 2 and 5% of hydrogen peroxide based on colloidal silver for 30 seconds, 45 seconds, 1, 2 and 5 minutes and 5 ml bleach and pure water (as control) were used to compare the process of decapsulation with the test solutions. After decapsulation, 1 gram of cysts per liter were hatchedat 30 ° C for 48 hours under conditions of 35 ppt salinity. After 48 hours of incubation, 99% hatching rate at a dose of 0.5 % solution of hydrogen based on colloid silver, 97% in bleach solution and 94 % in the control cysts was observed. According to the results of the statistical analysis, ANOVA, significant difference was found between the results of test treatments. The results of this study suggest that, best performance of hatching rate in Artemia urmiana cysts can be achieved, by putting the cysts in a solution with a dose of 0.5% of hydrogen peroxide based on silver colloid.
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