A study on effects of isoflavones of Soya oil on growth and maturation of Oocytes of unmature female three-spot Gourami(Trichogaster trichopterus)

Subject Areas : Marine Biology

T. Naji ¹ , H. Hosseinzadeh Sahafi ² , M. K. Jazebizadeh ³ , Z. Samari ⁴

1 - Pharmaceutical Sciences Branch,Islamic Azad University
2 - Fisheries Research Organization
3 - Pharmaceutical Sciences Branch,Islamic Azad University
4 - Pharmaceutical Sciences Branch,Islamic Azad University

Received: 2010-06-30 Accepted : 2010-09-03 Published : 2010-12-21

Keywords: Ovary, Trichogaster trichopterus, Soya oil, isoflavoes,

Abstract :

The effects of Soya oil isoflavones on growth and maturation of Oocytes in unmature Trichogaster trichopterus female is studied. After declorization of aquarium water, unmature female fish specimens with weight of 1.5- 2.5 g were placed in water. The experiments were done in seven treatment (two control treatment and five main treatment),each treatment invoved 10 fish specimens. Main fish treatments received 5 doses of Soya oil with 10, 20, 30, 40 & 50 µg/kg. Injection were intra muscular under dorsal fin of fishes. Seven days after injection fish specimens were kiled, their ovaries were removed and fixed in10% formalin. The routine procedures of preparation of tissue were fpllowed and the paraffin bloks were cut at 5-7 micros, stained with H&E. Then, the mean of gonadosomatic index, frequency percentage of matured and unmatured ovaries, the mean of small and big diameter of mature and unmatured ovaries and histological structures of ovary were compared with control treatments,gonadosomatic index in controls were 5.7% and in the fifth treatment it was 6.7% .Also with increased dose ,frequency percentage of matured and diameter of mature ovaries increased. Results showed the application of Soya oil isoflavones can induce final maturation in Three spot gourami and most effective was more doses of Soya oil (p<0/05).