Antibacterial activity and chemical profiling of Bombax glabrum (Pasq.) A. Robyns leaves
Subject Areas : Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)
اولوفونمی
وسو
1
(گروه داروسازی و شیمی دارویی ، دانشگاه Olabisi Onabanjo Ago-Iwoye ، نیجریه ؛)
پل
اوگانباموو
2
(مرکز تحقیقات زیست پزشکی ، موسسه تحقیقات جنگلداری نیجریه ؛)
ساموئل
بانکول
3
(پلی تکنیک فدرال ایل-اولوجی ، ایالت اوندو ، نیجریه ؛)
اولامیلکان
آتدو
4
(مرکز تحقیقات زیست پزشکی ، موسسه تحقیقات جنگلداری نیجریه ؛)
روفیات
ائیدیران
5
(مرکز بیوتکنولوژی ، موسسه تحقیقات جنگلداری نیجریه ؛)
Keywords: GC-MS, phytochemicals, Antibacterial activity, Column chromatography, Bombax glabrum, TLC,
Abstract :
Background & Aim:Bombax glabrum is used in traditional medicine for the relief of general pain and digestive problems. The aim of this study was to establish the antibacterial activity, to characterize and identify the bioactive compounds in the leaves of the plant.Experimental: The powdered leave samples were sequentially extracted with n-hexane and chloroform using a soxhlet apparatus. Phytochemical screening was done using standard analytical procedures and the antibacterial activity of both extracts was tested against Bacillus subtills, Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia using modified agar disc diffusion method. The chloroform extract was subjected to column chromatography separation in combination with TLC. Contact autobiography was carried out on two active spot(s) while purified fractions of these spots were analyzed using GC-MS.Results: Phytochemical screening shows the presence of saponins, flavonoids, tannins, alkaloids, and sterols. The plant has inhibitory activity against S. aureus and E. coli; while the GC-MS of two active spots of the chloroform extract (with Rf of 0.60 and 0.82) yielded 12 and 17 compounds for band C3 and C5, respectively, which were active against S. aureus only. Most abundant compounds are bis (2-ethylhexyl) phthalate (34.54) and 2-pentadecanone, 6,10,14-trimethyl (41.81 %), respectively.Recommended applications/industries: The presence of bioactive compounds could account for the plant’s inhibitory action against S. aureus and E. coli which may justify its use in managing infections
Abdu, K. and Ammani, H. 2017. Phytochemical and antibacterial screening of the leaf extracts of Bombax buonopozense. Chemistry Research Journal, 2(3): 50-55.
Adeleke, R.O. and Abiodun, O.A. 2010. Nutritional and physico-chemical properties of Bombax glabrum seeds. Pakistan Journal of Nutrition, 9(9): 856-857.
Adeosun, T.E., Ogunwande, I.A., Avoseh, O.N., Raji, I.P. and Lawal, O.A. 2017. Composition and anti-inflammatory activity of essential oil of Jatropha curcas. Natural Product Communications, 12(3): 439-440.
Akuodor, G., Essien, A., Ibrahim, J., Bassey, A., Akpan, J., Ikoro, N. and Onyewenjo, S. 2012. Phytochemical and antimicrobial properties of the methanolic extracts of Bombax buonopozense leaf and root. Asian Journal of Medical Sciences, 2(3): 190-194.
Amudha, P., Jayalakshmi, M., Pushpabhrathi, N. and Vanitha, V. 2018. Identification of bioactive components in Enhalus acoroides seagrass extract by gas chromatography-mass spectrometry. Asian Journal of Pharmaceutical and Clinical Research, 11(10): 313-315.
Araujo, F.D.S., Cavalcante, A.N., Sousa, M.D.B., Moura, C.V.R., Chaves, M.H., Aued-Pimentel, S. and Chaves, S.K.M. 2017. Biodiesel production from Bombacopsis glabra oil bymethyl transesteriļ¬cation method. Energies, 10: 1360.
Arora, S., Kumar, G. and Meena, S. 2017. Screening and evaluation of bioactive components of Censhrus ciliaris L. by GC-MS analysis. International Research Journal of Pharmacy, 8(6): 69-76.
Baris, O., Gulluce, M., Sahin, F., Ozer, H., Kilic, H., Ozkan, H. and Ozbek, T. 2006. Biological activities of the essential oil and methanol extract of Achilea biebersteinii Afan. (Asteraceae). Turkish Journal of Biology, 30: 65-73.
Chaves, M.H., Araujo, F.D.S., Moura, C.V.R., Tozetto, L.J., Aued-Pimentel, S. and Caruso, M.S.F. 2012. Chemical characterization and stability of the Bombacopsisglabranut oil. Food and Public Health, 2(4): 104-109.
Doughari, J.H. and Ioryue, A.S. 2009. Antimicrobial activity of stem bark extracts of Ceibapentandra. Pharmacologyonline, 1(1): 1333-1340.
El-Sayed, O., Asker, M., Shash, S. and Hamed, S.R. 2015. Isolation, structure elucidation and biological activity of di-(2-ethylhexyl) phthalate produced by Penicillium janthinellum. International Journal of Chemical Technology Research, 8(1): 58-66.
Kamble, M.A., Mahapatra, D.K., Dhabarde, D.M., Ingole, A.R. 2017. Pharmacognostic and pharmacological studies of Bombax ceibat horn extract. Journal of Pharmacy and Pharmacognosy Research, 5(1): 40-54.
Kasim, L.S., Ferro, V., Odukoya, O.A., Ukpo, G.E., Seidel, V., Gray, A.I. and Waigh, R. 2011. Cytotoxicity of isolated compoundsfrom the extracts of Struchium sparganophora (linn) Ktze Asteraceae, Parkistan Journal of Pharmaceutical Science, 24(4): 475-478.
Krishnananda, P.I., Amit, G.D., Dipika, A.P., Mahendra, S.D., Mangesh, P.M. and Vaibhav C.K. 2017. Phytochemicals: extraction methods, identification and detection of bioactive compounds from plant extracts. Journal of Pharmacognosy and Phytochemistry, 6(1): 32-36.
Lawal, O.A., Ogunwande, I.A., Salvador, A.F., Sanni, A.A. and Opoku, A.R. 2014. Pachira glabra Pasq. essential oil: chemical constituents, antimicrobial and insecticidal activities. Journal of Oleo Science, 63(6): 629-635.
Naidoo, Y., Sadashiva, C.T., Kasim, N., Nicholas, A. and Naidoo, G. 2014. Chemical composition and antimicrobial activity of essential oil of Ocimum obovatum E. Mey. Ex Benth. (Lamiaceae). Journal of Essential Oil Bearing Plants, 17(1): 142-147.
Ogunlade I., Ilugbiyin A. and Osasona A.I., 2011. A comparative study of proximate composition, antinutrient composition and functional properties of Pachira glabra and Afzelia Africana seed flours. African Journal of Food Science, 5(1): 32-35.
Oni, P.I., Malomo, O. and Adekoyeni, O.O. 2015. Preliminary evaluation of the ecology, economic importance and nutritional potentials of Pachira glabra (Pasq.); a neglected fruit tree in Nigeria. International Journal of Current Microbiology and Applied Sciences, 4(2): 1030-1036.
Osuntokun, O.T., Ayodele, A.O., Adeoye, M.I. and Odufunwa, A.E. 2017. Assessment of antimicrobial and phytochemical properties of crude leaf and bark extracts of Ceiba pentandra on selected clinical isolates found in Nigerian teaching hospital. Journal of Bacteriology & Mycology: Open Access, 4(1): 00079.
Parulekar, G.T. 2017. Antibacterial and phytochemical analysis of Ceiba pentandra (L.) seed extracts. Journal of Pharmacognosy and Phytochemistry, 6(3): 586-589.
Paula, V.F., Barbosa, L.C.A., Errington, W., Howarth, O.W. and Cruz, M.P. 2002. Chemical constituents from Bombacopsis glabra (Pasq.) A. Robyns: complete 1H and 13C NMR assignments and X Ray structure of 5-Hydroxy-3,6,7,8,4'-pentamethoxy flavone. Journal of the Brazilian Chemical Society, 13(2): 276-280.
Refaat, J., Desoky, S.Y., Ramadan, M.A. and Kamel, M.S. 2013. Bombacaceae: A phytochemical review. Pharmaceutical Biology, 51(1): 100-130.
Rehman, R.U., Akhtar, N. and Mustafa, R. 2017. Antibacterial and antioxidant potential of stem bark extract of Bombax ceiba collected locally from south Punjab area of Pakistan. African Journal of Traditional Complementary and Alternative Medicine, 4(2): 9-15.
Sasidharan, S., Chen, Y., Saravanan, D., Sundram, K.M. and Yoga Latha, L. 2011. Extraction, isolation and characterization of bioactive compounds from plants' extracts. African Journal of Traditional Complementary Alternative Medicines, 8(1): 1-10.
Shah, S.S., Shah, S.S., Iqbal, A., Ahmed, S., Khan, W.M., Hussain, S. and Li, Z. 2018. Phytochemical screening and antimicrobial activities of red silk cotton tree (Bombax ceiba L.). Parkistan Journal of Pharmaceutical Sciences, 31(3): 947-952.
Soetan, K.O., Oyekunle, M.A., Aiyelaagbe, O.O. and Fafunso, M.A. 2006. Evaluation of the antimicrobial activity of saponins extract of Sorghum bicolorL. Moench. African Journal of Biotechnology, 5(23): 2405-2407.
Tagousop, C.N., Tamokou, J., Kengne, I.C., Ngnokam, D. and Voutquenne-Nazabadioko, L. 2018. Antimicrobial activities of saponins from Melanthera elliptica and their synergistic effects with antibiotics against pathogenic phenotypes. Chemistry Central Journal, 12: 97.
Trease and Evans W.C. 2008.Trease and Evans Pharmacognosy. WB. Saunders Company Limited, Nottingham 139.
Valle, D.L., Puzon, J.J.M., Cabrera, E.C. and Rivera, W.L. 2016. Thin layer chromatography-bioautography and gas chromatography-mass spectrometry of antimicrobial leaf extracts from Philippine Piper betle L. against multidrug-resistant bacteria. Evidence-Based Complementary and Alternative Medicine, 2016: 4976791.
Wang, Y., Wang, T., Ban, Y., Shen, C., Shen, Q., Chai, X., Zhao, W and Wei, J. 2018. Di-(2-ethylhexyl) phthalate exposure modulates antioxidant enzyme activity and gene expression in juvenile and adult Daphnia magna. Archives of Environmental Contamination and Toxicology, 75: 145-156.
Wintola, O.A. and Afolayan, A.J. 2017. Chemical constituents and biological activities of essential oils of Hydnora africana Thumb used to treat associated infections and diseases in South Africa. Applied Sciences, 7: 443-456.