Pharmacognostic study, chemical analysis and antioxidant potential of Leucas indica L. (R. Br.)
Subject Areas : Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)جاشری جاسوتکار 1 , راپالی شیرسات 2 , دیپاک کوچی 3
1 - Department of Botany, Shri Shivaji College of Arts, Commerce and Science, Akola (MS), India;
2 - Department of Botany, Dr. R. G. Rathod Arts and ,Science College, Murtizapur, Dist- Akola (MS) India;
3 - Department of Botany,
Shri Shivaji College of Arts, Commerce and Science, Akola (MS), India;
Keywords: Antioxidant, Phytochemistry, Flavonoids, HPTLC, phenolics, Leucasindica L. (R. Br.),
Abstract :
Background & Aim: Leucas indica L. (R. Br.) is a lesser known aromatic herb of family Lamiaceae. Traditionally it was use to cure cough and cold by tribals of Southern India. The present work was aimed to investigate its pharmacognostic characters, major phytochemicals and antioxidant potential as possible replacement of its allied members to reduce their exploitation. Experimental: The fine details of stem and leaves of L. indica were noted including vasculature, cortical and epidermal depositions, trichome structure and stomata. The fluorescence analysis of leaf powder was done using various laboratory chemicals and reagents. The methanol leaf extract was analyzed using HPTLC to identify major active compounds and antioxidant potential was studied using standard method. Results: Both simple and glandular trichomes were present on stem as well as leaves. The fluorescence analysis of powdered drug material after interaction with different laboratory chemicals showed distinct colorations. The preliminary analysis revealed the phytochemical richness of the plant. Most phytoconstituents were found to be extracted in methanol solvent. The HPTLC analysis of methanol extract showed presence of a range of phenolics and flavonoids. The chromatogram showed availability of rutin, ferulic acid, catechin and apigenin which makes this plant as a possible antioxidant drug candidate. Recommended applications/industries: The rich chemical diversity of this plant and significant antioxidant potential could be used as good natural source for herbal pharmaceuticals.
Ai, L. C., Jeyanthi, J.A.J. and Shreenivasan, S. 2012. Antioxidant and antibacterial activity of Leucas aspera. Asian Pacific Journal of Biomedicine, 2(3): 176-180.
Ali, M.S., Sayeed, M.A., Nabi, M.M. and Rahman, M.A.A. 2013. In vitro antioxidant and cytotoxic activities of methanol extract of Leucas aspera leaves. Journal of Pharmaognosy and Phytochemistry. 2(1): 8-13.
Blois, M.S. 1958. Antioxidant determinations by the use of a stable free radical. Nature, 29: 199-200.
Chase, C.R. and Pratt, R.F. 1949. Fluorescence of powdered vegetable drugs with particular reference to development of system of identification. Journal of American Pharma Association., 38: 324-333.
Harborne, J.B. 1973. Phytochemical methods, Chapman and hall, ltd, London, Pp. 49-188.
Itoria, P., Jain, S., Jain, G. and Dubey, B.K. 2011. Pharmacognostic evaluation and phytochemical screening of Leucascephalotes. International Journal of Phytopharmacy. 1(1): 15-26.
Kahkonen, M.P., Hopia, A.I., Vuorela, H.J., Rauha, J., Pihlaja, K., Kujala, T.S. and Heinonen, M. 1999. Antioxidant activity of plant extracts containing phenolic compounds. Journal of Agriculture and Food Chemistry, 47: 3954-3962.
Krishanaiah, D., Devi, T., Bano, A. and Sarbatly, R. 2009. Studies on phytochemical constituents of six Malaysian medicinal plants. African Journal of Medicinal Plant Research, 3(2): 67-72.
Makhija, I.K., Chandrashekar, K.S., Richard, L. and Jaykumar, B. 2011. Phytochemical and Pharmacological profile of Leucas lavandulaefolia: A review. Research Journal of Medicinal Plants, 5(5): 500-507.
Menon, D.B., Sashikumar, J.M. and Latha, K. 2012. Phytochemical analysis and antioxidant activity of methanolic extract of Plectranthus hadiensis (Forssk.) Scheinf. Ex. Spreng. areal parts. Indian Journal of Natural Products and Resources. 3(3): 359-365.
Naik, V.N. 1998. Flora of Marathwada, Amrut Prakashan, Aurangabad (MS).
Paul, S. and Saha, D. 2012. Pharmacognostic studies on methanolic extract of areal part of L. zeylanica (L.). Asian Journal Pharmaceutical Research, 2(4): 142-143.
Sudhakara Reddy, M., Subramanyam, P., Sivasankar, R. Subba Reddy, S.V., Udaykiran, V. and Subahan, M. 2017. Phytochemical screening of Leucas aspera and their anti-inflammatory activity. International Journal of Advanced Scientific and Technical Research, 7(4): 40-57.
Tahareen, S., Shwetha, R. andMyrene, R.D. 2016. Potential antioxidant, anti inflammatory and antibacterial evaluation of extracts of Leucas aspera using in vitro models. International Journal of Pharmacy and Pharmaceutical Science, 8(11):292-297.
Udayan, P.S., Tushar, K.V., George, S. and Balachandran, I. 2007. Ethnomedicinal information from Kattunayakas tribes of Mudumalai Wildlife Sanctuary, Nilgiris district, Tamil Nadu. Indian Journal of Traditional Knowledge, 6: 574-578.