Study on gentic diversity of the honey bee, Apis mellifera L. using microstatellite ISSR markers in three provinces of Iran

Subject Areas : entomology and othea arthropods

N. Palvaneh ¹ , E. Soleyman-Nejadian ² , R. Radjabi ³ , F. Yaghout nejad ⁴

1 - Department of Entomology, Faculty of Agriculture, Islamic Azad University Arak Branch, Arak, Iran
2 - Department of Entomology, Faculty of Agriculture, Islamic Azad University, Arak Branch, Arak, Iran
3 - Plant Protection Department, Faculty of Agriculture, Islamic Azad University Dezful Branch, Dezful, Iran
4 - Department of Entomology, Faculty of Agriculture, Islamic Azad University, Arak Branch, Arak, Iran

Received: 2013-11-12 Accepted : 2014-12-17 Published : 2015-11-22

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Abstract :

Microsatellite markers ISSR were used for isolation of Apis mellifera L. from populations of Kurdistan, Khuzestan and Esfahan. DNA was extracted from worker bees by optimized salting method. Reproducing was done by 4 pairs of ISSR Primers. The obtained bands were visible using 2% agaroz gel & satining with ethidium bromaid. The Bands pattern was recorded by presence (1) or absence (0) method. Total of 45 bands were observed which of 84.52 were diffrent.The cluster analysis based on jaccard similarity cofficient indicated the genetic distance. Populations of  Kurdistan & Khuzestan were located in group (I) and Esfahan in group (II). Therefore, populations of  Kurdistan and Khuzestan provinces showed the highest similarity.