Comparison of oocyte quality from normal mouse and mouse with polycystic ovarian syndrome following evaluation of GDF-9 gene
Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms
Fatemeh Kousheh
1
,
Fatemeh Ghasemian
2
1 - Department of Biology, Faculty of Sciences, University of Guilan, Namjoo St., Rasht, Iran
2 - Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran
Keywords: Polycystic ovarian syndrome, GDF-9, Oocyte maturation, Oocyte quality,
Abstract :
Introduction: Polycystic ovarian syndrome (PCOS) is one of the most common causes of infertility. One of assisted reproductive methods for PCOS patients is using of in-vitro maturation (IVM). Normal GDF-9 gene expression is one of the symptoms of good oocyte quality. This study examines the differences between quality of PCOS oocytes/follicles with normal oocytes/follicles during IVM.Materials and Methods: Induction of PCOS in NMRI mice (n=10) was performed by injection of 4mg/kg estradiol valerate dissolved in 0.2mg sesame oil once daily for 60 days. Oocytes and follicles were collected from control and PCOS groups, and cultured in the MEM-α medium supplemented with 10% FBS for 24-48 hrs. The evaluation of IVM rate and GDF-9 gene expression (Real-Time PCR method) was performed in different groups. Statistical analysis was performed using one-Way ANOVA test.Results: In the PCOS group, a significant decrease was observed in the number of primary follicles, preantral, antral, and corpus luteum. Increased cystic follicles and follicles with degenerated granulosa layer were also observed. 52% and 50% of PCOS oocytes and follicles was matured, respectively. In the control group, 83.33% of oocytes and 75% of follicles matured (P<0.05). GDF-9 gene expression was 1.00±0.08 and 1.24±0.2 in normal oocytes and follicles (P<0.01) and 0.66±0.02 and 0.37±0.02 in PCOS oocytes and follicles, respectively (P<0.001). Conclusion: Quality of PCOS oocytes, and especially, follicles (GDF-9 gene expression) was decreased following IVM. Therefore, using of PCOS oocytes was suggested for IVM
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