Investigation of the effect of Deferoxamine preconditioning on VEGF, ANGP1, and TFGβ1 gene expression in diabetic adipose derived stem cells (ADSCs)

Subject Areas : Journal of Comparative Pathobiology

راضیه Tajali ¹ , اکرم Eidi ² , حسین Ahmadi Tafti ³ , عبدالرضا Pazouki ⁴ , A.M Sharifi ⁵

1 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
3 - Research Center for Advanced Technologies in Cardiovascular Medicine, Tehran Heart Center Hospital, Tehran University of Medical Sciences, Tehran, Iran.
4 - Minimally Invasive Surgery Research Center, Iran University of Medical Sciences Tehran, Iran
5 - - Stem Cell and Regenerative Medicine Research Center, Iran University of Medical Sciences, Tehran, Iran. -Razi Drug Research Center, and Department of Pharmacology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. -Tissue Engineering Group, (NOCERAL), Department of Orthopedics Surgery, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia

Received: 2023-05-24 Accepted : 2023-08-06 Published : 2023-08-23

Keywords: Adipose tissue-derived stem ce, Deferoxamine, Angiogenesis,

Abstract :

Adipose tissue-derived stem cells (ADSCs) have been considered a promising treatment for diabetic wound repair. However, the effectiveness of autologous ADSC-derived therapy for diabetic patients needs to be improved. The aim of this study was to investigate the effect of pretreatment with deferoxamine on the expression of the angiogenic genes in diabetic ADSCs preconditioned with deferoxamine. In this experimental study, abdominal adipose tissue was taken from three patients with type 2 diabetes and three healthy individuals, and stem cells derived from adipose tissue were extracted by the enzymatic method. Diabetic ADSCs were treated with 75, 150, and 300 μM deferoxamine concentrations for 24 and 48 hours. Then, the cell survival rate was evaluated using the WST1 test and the expression level of VEGF, ANGP1, and TFGβ1 genes in normal, diabetic, and diabetic ADSCs treated with deferoxamine using the Real-time PCR technique. The data were analyzed using Prism statistical software. Our results showed that the expression levels of VEGF, ANGP1 and TGFβ1 genes in diabetic samples were significantly decreased compared to the normal group (P<0.05). However, the expression level of all three genes after treating the cells with concentrations of 150 and 300 μM deferoxamine for 24 hours was significantly increased compared to the untreated diabetic group (P<0.05). Our results showed that deferoxamine significantly increases the expression of VEGF, ANGP1, and TGFβ1 in diabetic ADSCs and increases their angiogenic capacity.

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