Rapid recognition of typhoid and non-typhoid Salmonella in poultry samples using multiple polymerase chain reaction method (multiplex-PCR)

Subject Areas : Journal of Comparative Pathobiology

A Khodadadeh Jigheh ¹ , Y Anzabi ²

1 - Graduate of master in microbiology. faculty of basic sciences. Tabriz branch. Islamic Azad university. Tabriz Iran
2 - -Associate Professor. department of pathobiology. faculty of veterinary medicine. Tabriz medical sciences. Islamic Azad university. Tabriz Iran-Department of Microbial Biotechnology, Biotechnology Research Center, Tabriz Branch, Islamic Azad University, Tabriz, Iran

Received: 2023-04-04 Accepted : 2023-06-20 Published : 2023-08-23

Keywords: Poultry, Typhoid Salmonella, Multiplex polymerase chain rea,

Abstract :

The Salmonella serotypes are detected mainly through traditional microbiologic methods, which are associated with problems. The invention of rapid molecular detection methods has somewhat resolved these problems. This study aimed to assess the possibility of rapid detection of typhoid and non-typhoid Salmonellas in poultry using the multiplex polymerase chain reaction (PCR). A total of 40 isolates of Salmonella from industrial poultry were collected veterinary laboratories in Tabriz, Iran. After microbiological and serological tests, we confirmed that 27 out of 40 isolates belonged to the Salmonella entrica species. Differential tests revealed that 15, 7, 2, and 3 isolates were Salmonella Gallinarum, Salmonella Enteritidis, Salmonella Typhimurium, and Salmonella Pullorum, respectively. We then used specific primers to multiply the genes invA, rfbJ, lygD, I137_08605, and speC. The 27 isolates were then genotypically analyzed through multiplex-PCR. The results showed that all 27 isolates and the standard strains of all 4 bacteria carry the invA gene, while this gene was absent in 13 non-Salmonella isolates. The I137_08605 genes was present in all isolates and the standard strains of S. Gallinarum and S. Pullorum; the rfbJ and lygD genes were present in all isolates of S. Enteritidis and S. Typhimurium and their standard strains; and the speC gene was present in all isolates of S. Gallinarum and some isolates of S. Typhimurium and S. Entritidis and their standard strains. It seems that typhoid Salmonellas of poultry, i.e., S. Gallinarum and S. Pullorum, can be discriminated from non-typhoid Salmonellas through the multiplex-PCR molecular method. This can prevent further damage to the country's poultry industry by preventing their further spread.

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