Antioxidant effect of copper nanoparticles coated with Artemisia annua plant extract in the 2nd degree burns in mice
Subject Areas : Journal of Comparative Pathobiology
فرزانه
Tavakoli
1
(Department of Basic Science, Faculty of Veterinary Medicine, Shahrekord University, shahrekord, Iran)
بهناز
Karimi babaahmadi
2
(Department of Basic Science, Faculty of Veterinary Medicine, Shahrekord University, shahrekord, Iran)
موسی
Javdani
3
(Department of Clinical Science, Faculty of Veterinary Medicine, Shahrekord University, shahrekord, Iran)
جهانگیر
Kaboutari
4
(Department of Basic Science, Faculty of Veterinary Medicine, Shahrekord University, shahrekord,)
Keywords: Second-degree burn, Artemisia annua, antioxidant, Copper Nanoparticles,
Abstract :
Burns trigger intricate inflammatory processes and the generation of free radicals. The anti-inflammatory, antioxidant, and antibacterial characteristics of the Artemisia annua plant have a significant potential for enhancing the healing process for wounds. This study examines the impact of an Artemisia annua extract-coated copper nanoparticle ointment on the antioxidant enzyme activity in second-degree burns in mice. After the green synthesis of coated copper nanoparticles, 100 male mice were divided into five groups: control without treatment, eucerin ointment group, 0.2% copper nanoparticle ointment, 0.2% copper nanoparticles coated with Artemisia annua extract ointment, and 5% Artemisia annua extract ointment. The activity of antioxidant enzymes and the level of lipid peroxidation in tissue homogenates were measured on days 14, 7, 3, and 21. SOD, CAT, and GPX activities were all significantly higher on day 7 in the coated copper nanoparticle ointment group compared to the control group (P˂0.05). The level of MDA was also significantly lower in the control group on days 7, 14, and 21 (P 0.05). The extract of the medicinal plant Artemisia annua is useful for making and coating copper nanoparticles. In addition to the advantages of plant extract, copper nanoparticles have a synergistic impact that speeds up the healing of second-degree burn wounds in mice by lowering lipid peroxidation in wound tissue and boosting antioxidant activity.
2. Palackic A, Jay JW, Duggan RP, Branski LK, Wolf SE, Ansari N, et al. Therapeutic Strategies to Reduce Burn Wound Conversion. Medicina. 2022;58(7):922.
3. Qiu X, Wu Y, Zhang D, Zhang H, Yu A, Li Z. Roles of Oxidative Stress and Raftlin in Wound Healing Under Negative-Pressure Wound Therapy. Clinical, Cosmetic and Investigational Dermatology. 2021:1745-53.
4. Singh BN, Prateeksha n, Upreti DK, Singh BR, Defoirdt T, Gupta VK, et al. Bactericidal, quorum quenching and anti-biofilm nanofactories: a new niche for nanotechnologists. Critical reviews in biotechnology. 2017;37(4):525-40.
5. Din MI, Arshad F, Hussain Z, Mukhtar M. Green adeptness in the synthesis and stabilization of copper nanoparticles: catalytic, antibacterial, cytotoxicity, and antioxidant activities. Nanoscale research letters. 2017;12:1-15.
6. Mazandarani M, Majidi Z, Zarghami-Moghaddam P, Abrodi M, Hemati H, Fathiazad F. Essential oil composition, total phenol, flavonoid, anthocyanin and antioxidant activities in different parts of Artemisia annua L. in two localities (North of Iran). Journal of Medicinal plants and By-product. 2012;1(1):13-21.
7. Wang KS, Li J, Wang Z, Mi C, Ma J, Piao LX, et al. Artemisinin inhibits inflammatory response via regulating NF-κB and MAPK signaling pathways. Immunopharmacology and immunotoxicology. 2017;39(1):28-36.
8. Katadj JK, Rafieian-Kopaei M, Nourani H, Karimi B. Wound healing effects of Artemisia sieberi extract on the second degree burn in mice skin. Journal of HerbMed Pharmacology. 2016;5(2):67-71.
9. Zangeneh MM, Ghaneialvar H, Akbaribazm M, Ghanimatdan M, Abbasi N, Goorani S, et al. Novel synthesis of Falcaria vulgaris leaf extract conjugated copper nanoparticles with potent cytotoxicity, antioxidant, antifungal, antibacterial, and cutaneous wound healing activities under in vitro and in vivo condition. Journal of Photochemistry and Photobiology B: Biology. 2019;197:111556.
10. Jridi M, Sellimi S, Lassoued KB, Beltaief S, Souissi N, Mora L, et al. Wound healing activity of cuttlefish gelatin gels and films enriched by henna (Lawsonia inermis) extract. Colloids and Surfaces A: Physicochemical and Engineering Aspects. 2017;512:71-9.
11. Feng X, Cao S, Qiu F, Zhang B. Traditional application and modern pharmacological research of Artemisia annua L. Pharmacology & therapeutics. 2020;216:107650.
12. Tao B, Lin C, Deng Y, Yuan Z, Shen X, Chen M, et al. Copper-nanoparticle-embedded hydrogel for killing bacteria and promoting wound healing with photothermal therapy. Journal of Materials Chemistry B. 2019;7(15):2534-48.
13.Hemmati S, Ahmeda A, Salehabadi Y, Zangeneh A, Zangeneh MM. Synthesis, characterization, and evaluation of cytotoxicity, antioxidant, antifungal, antibacterial, and cutaneous wound healing effects of copper nanoparticles using the aqueous extract of Strawberry fruit and L-Ascorbic acid. Polyhedron. 2020;180:114425.
14. Ghosh S, More P, Nitnavare R, Jagtap S, Chippalkatti R, Derle A, et al. Antidiabetic and antioxidant properties of copper nanoparticles synthesized by medicinal plant Dioscorea bulbifera. Journal of Nanomedicine & Nanotechnology. 2015(S6):1.
15. Sandoval C, Ríos G, Sepúlveda N, Salvo J, Souza-Mello V, Farías J. Effectiveness of Copper Nanoparticles in Wound Healing Process Using In Vivo and In Vitro Studies: A Systematic Review. Pharmaceutics. 2022;14(9):1838.
16. Ognik K, Stępniowska A, Cholewińska E, Kozłowski K. The effect of administration of copper nanoparticles to chickens in drinking water on estimated intestinal absorption of iron, zinc, and calcium. Poultry science. 2016;95(9):2045-51.
17. Mirghaed AT, Paknejad H, Mirzargar SS. Hepatoprotective effects of dietary Artemisia (Artemisia annua) leaf extract on common carp (Cyprinus carpio) exposed to ambient ammonia. Aquaculture. 2020;527:735443.
18. Chukwurah PN, Brisibe EA, Osuagwu AN, Okoko T. Protective capacity of Artemisia annua as a potent antioxidant remedy against free radical damage. Asian Pacific journal of tropical biomedicine. 2014;4:S92-S8.
19. Kim MH, Seo JY, Liu KH, Kim J-S. Protective effect of Artemisia annua L. extract against galactose-induced oxidative stress in mice. PloS one. 2014;9(7):e101486.
20. Dawood MA, Eweedah NM, Moustafa EM, El-Sharawy ME, Soliman AA, Amer AA, et al. Copper nanoparticles mitigate the growth, immunity, and oxidation resistance in common carp (Cyprinus carpio). Biological Trace Element Research. 2020;198:283-92.
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