An experimental study of histopathological lesions of secondary infection with H9N2 avian influenza virus following inoculation by Ornithobacterium rhinotracheale in SPF chickens

Subject Areas : Journal of Comparative Pathobiology

آیدین  Azizpour ¹ (Department of Medicinal Plants, University of Mohaghegh Ardabili, Ardabil, Iran)
زهرا  Amirajam ² (Department of Cardiology, Ardabil University of Medical Sciences, Ardabil, Iran)
راحم  , khoshbakht ³ (Department of Pathobiology, Amol University of Special Modern Technologies, Amol, Iran)
نورالدین  Hosseinpour Azad ⁴ (Department of Medicinal Plants, University of Mohaghegh Ardabili, Ardabil, Iran)

Received: 2023-02-12 Accepted : 2023-04-24 Published : 2023-05-22

Keywords: ORT, H9N2 virus, Secondary infection, Histopathological lesions, SPF chickens,

Abstract :

The purpose of this study was to evaluate the type, severity and extent of tissue lesions induced by secondary infection with avian influenza virus following inoculation by ORT in SPF chickens. Sixty, one-day-old SPF chicks were provided and divided randomly into three groups. At the age of 21 days, the chicks in the first group were inoculated only with ORT and second group inoculated first with ORT and three days later with H9N2 virus. Each bird in third group was received only with PBS as control. Tissue samples were taken from trachea, lungs, liver, kidneys, bursa of fabricius, thymus, spleen and cecal tonsils for histopathological studies and after preparation and tissue sections were stained by Hematoxylin and Eosin (H&E) method. In chickens of the first group, ORT bacteria in addition to cause tissue lesions in organs of the respiratory system (loss of cilia with infiltration of mononuclear cells under the mucosa in the trachea, hyperemia and pneumonia in the lung), liver (congestion of sinusoids and central veins) and the kidneys (Hemorrhage and necrosis of urinary tubes) in  immune system organs also cause tissue lesions such as lymphocyte depletion in the bursa of fabricius, congestion and heterophil infiltration in thymus and increase in the of lymphoid follicles in the spleen and cecal tonsils. The histopathological lesions in chickens of the second group was more severe and longer than that of the first group. Tissue changes were not observed in control group. The results of this study showed that in chickens inoculated by ORT and under secondary infection with avian influenza virus cause exacerbate and prolongation histopathological lesions induced by ORT.

References:

1. Chin RR. Van Empel PCM, Hafez HM. Ornithobacterium rhinotracheare infection. In: Swayne DE (Ed.). Disease of Poultry, 13th edition: Wiley-Blackwell: Ames, NJ, USA. 2013: 807–858.


2. Goudarzi H, Azizpour A, Banani M, Nouri A, Seifi S. Survey of tissue tropism and dissemination of ORT-R87-7/1387 strain of Ornithobacterium rhinotracheale in SPF chickens. Veterinary Researches & Biological Products. 2015;28(2):2-8.


3. Banani M, Khaki P, Goodarzi H, Yousefi J, Pourbakhsh S. Isolation and identification of Ornithobacterium rhinotracheale from a broiler and a pullet flock. Pajouhesh-va-Sazandegi. 2000; 46: 106-109.


4. Van Empel P, Hafez H. Ornithobacterium rhinotracheale: a review. Avian pathology. 1999;28(3):217-27.


5. Banani M, Momayez R, Pourbakhsh S, Goodarzi H, Bahmani Nejad MA. Simultaneous isolation of O. rhinotracheale and avian influenza virus subtype H9N2 from commercial poultry chickens. Iranian Journal of Veterinary Research. 2002; 6: 190-195.


6. Azizpour A, Goudarzi H, Nouri A, Seifi S, Bijanzad P. A study of experimental infection with Ornithobacterium rhinotracheale on pathogenesis of avian influenza virus H9N2 subtype. Journal of Veterinary Microbiology. 2016; 12 (2): 23-33.


7. Goudarzi H, Azizpour A, Charkhkar S, Hablolvarid M, Momayez R. Experimental study of histopathological lesions induced by ornithobacterium rhinotracheale infection followed inoculation with H9N2 avian influenza virus. Journal of Comparative Pathobiology. 2016; 12(4) : 1783-1792.


8. Pan Q, Liu A, Zhang F, Ling Y, Ou C, Hou N, et al. Co-infection of broilers with Ornithobacterium rhinotracheale and H9N2 avian influenza virus. BMC veterinary research. 2012;8(1):1-7.


9. Thachil A, Velayudhan BT, Shaw DP, Halvorson DA, Nagaraja KV. Pathogenesis of Ornithobacterium rhinotracheale in egg-laying hens with coexisting infectious bronchitis virus and Escherichia coli infections. Journal of Applied Poultry Research. 2009;18(4):780-8.


10. Azizpour A, Goudarzi H, Charkhkar S, Momayez R, Hablolvarid M. Experimental study on tissue tropism and dissemination of H9N2 avian influenza virus and Ornithobacterium rhinotracheale co-infection in SPF chickens. Journal of Animal and Plant Sciences. 2012;8(1):1-7.


11. Azizpour A, Goudarzi H, Charkhkar S, Momayez R, Hablolvarid M, Bijanzad P. An experimental study of histopathological lesions caused by H9N2 subtype of avian influenza virus and Ornithobacterium rhinotracheale co-infection. Journal of Veterinary Research. 2018;73(2( : 199-207.


12. Hablolvarid MH, Sohraby Haghdost I, Pourbakhsh S, Gholami M. A study on histopathologic changes in chicken following intravenous inoculation with avian influenza virus A/Chicken/Iran/259/1998 (H9N2). Archives of Razi Institute. 2003; 55(1): 41-54.


13. Costa-Hurtado M, Afonso CL, Miller PJ, Spackman E, Kapczynski DR, Swayne DE, et al. Virus interference between H7N2 low pathogenic avian influenza virus and lentogenic Newcastle disease virus in experimental co-infections in chickens and turkeys. Veterinary research. 2014;45(1):1-11.

14. Swayne DE, Suarez LS, Sims LD. Avian influenza. In: Swayne DE (Ed.). Disease of poultry,13th edn: Wiley-Blackwell: Ames, USA. 2013: 181–218.


15. Chu J, Zhang Q, Zuo Z, El-Ashram S, Guo Y, Zhao P, et al. Co-infection of Chlamydia psittaci with H9N2, ORT and Aspergillus fumigatus contributes to severe pneumonia and high mortality in SPF chickens. Scientific reports. 2017;7(1):13997.


16. Morales-Erasto V, Falconi-Agapito F, Luna-Galaz G, Saravia L, Montalvan-Avalos A, Soriano-Vargas E E, et al. Coinfection of Avibacterium paragallinarum and Ornithobacterium rhinotracheale in chickens from Peru. Avian Diseases. 2016;60(1):75-78.


17. Zhao P, Wu G, Zhang Q, Chu J, Xie C, Wang Y, et al. Experimental Investigation on Ornithobacterium rhinotracheale and Enterococcus faecalis Co-Infection in Chickens. Pakistan Veterinary Journal. 2015;35 (2): 173-177.


18. Mo IP, Brugh M, Fletcher OJ, Rowland GN, Swayne DE. Comparative Pathology of Chickens Experimentally Inoculated with Avian Influenza Viruses of Low and High Pathogenicity. Avian Diseases. 1997;41(1):125-36.


19. Pourbakhsh SA, Sohraby Haghdost I, Hablolvarid MH, Gholami MR. Histopathological Study of Intratracheally Inoculated A/Chicken/Iran/259/1998 (H9N2) Influenza Virus in Chicken. Archives of Razi Institute. 2004;58(1):51-62.


20. Swayne DE, Slemons RD. Renal Pathology in Specific-Pathogen-Free Chickens Inoculated with a Waterfowl-Origin Type A Influenza Virus. Avian Diseases. 1990;34(2):285-94.


21. Slemons RD, Swayne DE. Replication of a Waterfowl-Origin Influenza Virus in the Kidney and Intestine of Chickens. Avian Diseases. 1990;34(2):277-84.


22. Swayne DE, Slemons RD. Comparative Pathology of a Chicken-origin and Two Duck-origin Influenza Virus Isolates in Chickens: The Effect of Route of Inoculation. Veterinary Pathology. 1994;31(2):237-45.


23. Bano S, Naeem K, Malik SA, editors. Evaluation of Pathogenic Potential of Avian Influenza Virus Serotype H9N2 in Chickens. Avian diseases; 2003 ; 2003; 47(3 Suppl): 817-822.


24. Pazani J, Marandi MV, Ashrafihelan J, Marjanmehr SH, Ghods F. Pathological studies of A/Chicken/Tehran/ZMT-173/99 (H9N2) influenza virus in commercial broiler chickens of Iran. International Journal of Poultry Science. 2008;7(5):502-10.