Molecular identification of Anaplasma ovis in sheep in Guilan province using PCR method

Subject Areas : Journal of Comparative Pathobiology

وحید  Noaman ¹ (Department of Parasitic Disease Research, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran)
نصرالله  Vahedi Nouri, ² (Department of Parasitic Disease Research, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran)
عبدالرضا  Nabinejad ³ (Department of Viral Poultry Disease Research, Research Razi Vaccine and Serum Research Institute, Agricultural)
هادی  Miranzadeh ⁴ (Instructor, Animal Science Research Department, Isfahan Agricultural and Natural Resources Research and Education Centre, Agricultural Research, Education and Extension Organization (AREEO), Isfahan, Iran.)
مسعود  Boroumand-Jazi ⁵ (Instructor, Animal Science Research Department, Isfahan Agricultural and Natural Resources Research and Education Centre, Agricultural Research, Education and Extension Organization (AREEO), Isfahan, Iran)

Received: 2022-08-09 Accepted : 2023-01-21 Published : 2023-01-21

Keywords: Molecular Identification, Anaplasma ovis, Sheep, Guilan, PCR,

Abstract :

Anaplasma ovis is the cause of disease in sheep and goats in many tropical and subtropical regions of the world. This study aimed to determine the Anaplasma ovis species in the sheep of Guilan province and the effect of some variables (season, age, and sex of the animal) on its prevalence. For this purpose, 200 blood samples were taken randomly from different parts of Guilan province through the veins of the sheep.  DNA extracted from blood samples was amplified with a primer pair that amplified a 1468 bp fragment of the 16S rRNA gene of the genus Anaplasma. Then the positive samples were amplified with the specific primer pair of Anaplasma ovis from the msp4 gene, which amplified the fragment of 866 bp. In total, 38 out of 200 samples (19%) were positive for Anaplasma ovis. In comparing the frequency of Anaplasma ovis in different seasons, the infection in the second six months of the year was significantly higher than in the first six months (P<0.05). However, no significant differences were observed in relation to the different ages of the investigated animals and the sex of animals (P>0.05). In this study, infection with Anaplasma ovis was confirmed in sheep in Gilan province. The study of the transmission methods of Anaplasma ovis and its vectors in Iran has remained largely unknown and requires more research in future studies.

References:

1. Ybañez AP, Sivakumar T, Ybañez RHD, Vincoy MRB, Tingson JA, Perez ZO, et al. Molecular survey of bovine vector-borne pathogens in Cebu, Philippines. Veterinary Parasitology. 2013;196(1-2):13-20.


2. Rymaszewska A, Grenda S. Bacteria of the genus Anaplasma–characteristics of Anaplasma and their vectors: a review. Vet Med. 2008;53(11):573-84.


3. Yasini S, Khaki Z, Rahbari S, Kazemi B, Amoli JS, Gharabaghi A, et al. Hematologic and clinical aspects of experimental ovine anaplasmosis caused by Anaplasma ovis in Iran. Iranian journal of parasitology. 2012;7(4):91.


4. Noaman V, Shayan P, Shahmoradi AH. Detection of Anaplasma ovis based on 16S rRNA gene by PCR-RFLP in sheep from central part of Iran. Journal of Veterinary Laboratory Research. 2009;1(1):27-37.


5. Noaman V, Bastani D, editors. Molecular study on infection rates of Anaplasma ovis and Anaplasma marginale in sheep and cattle in West-Azerbaijan province, Iran. Veterinary research forum; 2016: Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.


6. Jalali S, Khaki Z, Kazemi B, Bandehpour M, Rahbari S, RaziJalali M, et al. Molecular detection and identification of Anaplasma species in sheep from Ahvaz, Iran. 2013.


7. Yousefi A, Rahbari S, Shayan P, Sadeghi-dehkordi Z, Bahonar A. Molecular detection of Anaplasma marginale and Anaplasma ovis in sheep and goat in west highland pasture of Iran. Asian Pacific Journal of Tropical Biomedicine. 2017;7(5):455-9.


8. Vahedi Noori N, Noaman V. Molecular Identification of Anaplasma pathogenic Species in Sheep in Mazandaran Province. Veterinary Researches & Biological Products. 2020;33(2):29-41.


9. Niaz S, Zia Ur Rahman IA, Cossío-Bayúgar R, Amaro-Estrada I, Alanazi AD, Khattak I, et al. Molecular prevalence, characterization and associated risk factors of Anaplasma spp. and Theileria spp. in small ruminants in Northern Pakistan. Parasite. 2021;28.


10. Ghaffar A, Ijaz M, Ali A, Farooqi SH, Rehman A, Ali MM, et al. First report on molecular characterization of anaplasmosis in small ruminants in Pakistan. Journal of Parasitology. 2020;106(3):360-8.


11. Zhou M, Cao S, Sevinc F, Sevinc M, Ceylan O, Ekici S, et al. Molecular detection and genetic characterization of Babesia, Theileria and Anaplasma amongst apparently healthy sheep and goats in the central region of Turkey. Ticks and tick-borne diseases. 2017;8(2):246-52.


12. Noaman V. Identification of hard ticks collected from sheep naturally infected with Anaplasma ovis in Isfahan province, central Iran. Comparative Clinical Pathology. 2012;21(3):367-9.


13. Noaman V, Abdigoudarzi M, Nabinejad AR. Abundance, diversity and seasonal dynamics of hard ticks infesting cattle in Isfahan province, central Iran. Archives of Razi Institute. 2017;72(1):15-21.


14. Noaman V. Discrimination between Anaplasma marginale and Anaplasma ovis by PCR-RFLP. World Appl Sci J. 2013;21(2):190-5.


15. Hakimi H, Sarani A, Takeda M, Kaneko O, Asada M. Epidemiology, risk factors, and co-infection of vector-borne pathogens in goats from Sistan and Baluchestan province, Iran. Plos one. 2019;14(6):e0218609.


16. Reghaissia N, Dahmane A, Boularias G, Ghalmi F, Azzag N. Epidemiological and Comparative Diagnostic Study of Spp. Infection in Goats from North-Eastern Algeria. Folia Veterinaria. 2020;64(3):61-74.


17. Vahedi Noori N, Abdi Goodarzi M, Kiasari MN. Evaluation of the species diversity and abundance of hard ticks (Family: Ixodidae) parasite of cattle and sheep in Mazandaran province. Veterinary Researches & Biological Products. 2015;28(1):58-64.

18. Said MB, Belkahia H, Alberti A, Zobba R, Bousrih M, Yahiaoui M, et al. Molecular survey of Anaplasma species in small ruminants reveals the presence of novel strains closely related to A. phagocytophilum in Tunisia. Vector-Borne and Zoonotic Diseases. 2015;15(10):580-90.


19. Belkahia H, Said MB, El Hamdi S, Yahiaoui M, Gharbi M, Daaloul-Jedidi M, et al. First molecular identification and genetic characterization of Anaplasma ovis in sheep from Tunisia. Small Ruminant Research. 2014;121(2-3):404-10.


20. Riaz M, Nazir MM, Tasawar Z, Ahmed AN, Ayaz MM, Akram Q, et al. Molecular epidemiology and prevalence of Theileria lestoquardi and Theileria ovis infection in goats infested with tick vectors from Multan, Pakistan. Journal of Medical Entomology. 2019;56(3):844-8.