In vitro Effect of Carotenoid Pigment of Rhodotorula glutinis on Staphylococcus Aureus Isolated from Bovine Mastitis

Subject Areas : Journal of Comparative Pathobiology

S. Naisi, ¹ , منصور Bayat, ² , , T Zahraei Salehi, ³ , بهاره Rahimian Zarif, ⁴ , R. Yahyaraeyat, ⁵

1 - Department of Pathobiology, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Department of Pathobiology, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, , Tehran, Iran
3 - Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
4 - Department of Microbiology, Faculty of Basic Sciences, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran
5 - Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Received: 2022-05-29 Accepted : 2022-09-06 Published : 2022-09-06

Keywords: Antimicrobial resistance, Mastitis, Rhodotorula glutinis, Staphylococcus aureus, Yeast,

Abstract :

Mastitis, as a most costly disease of dairy cows, causes a lot of damage to the livestock industry in the world every year. One of the most important bacteria which causes clinical mastitis in cattle is S. aureus, which has become resistant to most common antibiotics in veterinary medicine. Therefore, researchers are looking for new methods in the treatment of such infections, in which the pigments of microorganisms fall into this category. The aim of this recent study was to optimize the production of carotenoid pigments by Rhododorula yeast and to investigate its antimicrobial effect on S. aureus isolates To isolate S. aureus, samples were taken from the milk of 100 cows with mastitis and the femA gene was identified for molecular confirmation among suspicious isolates of Staphylococcus by PCR method. Also, the isolate of R. glutinis obtained from one of the samples of mastitis was further examined and used as a sample of yeast producing pigment. The results showed that in addition to S. aureus as one of the causative agents of mastitis in dairy cattle, but also the yeast R. glutinis can be a causative agent of mastitis. The inhibitory effect on the growth of Staphylococcus using the pigment extracted from R. glutinis was also quite evident and more than 80% of S. aureus isolates were sensitive to the pigment at a concentration of 200 μg. R. glutenis inhibits excellent growth in S. aureus as one of the leading causes of mastitis in cows. The cause is the appearance of synthetic antibiotics and the reduction of microbial resistance.

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