In vitro Effect of Carotenoid Pigment of Rhodotorula glutinis on Staphylococcus Aureus Isolated from Bovine Mastitis

Subject Areas : Journal of Comparative Pathobiology

S. Naisi, ¹ (Department of Pathobiology, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran)
منصور Bayat, ² (Department of Pathobiology, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, , Tehran, Iran)
, T Zahraei Salehi, ³ (Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.)
بهاره Rahimian Zarif, ⁴ (Department of Microbiology, Faculty of Basic Sciences, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran)
R. Yahyaraeyat, ⁵ (Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran)

Received: 2022-05-29 Accepted : 2022-09-06 Published : 2022-09-06

Keywords: Antimicrobial resistance, Mastitis, Rhodotorula glutinis, Staphylococcus aureus, Yeast,

Abstract :

Mastitis, as a most costly disease of dairy cows, causes a lot of damage to the livestock industry in the world every year. One of the most important bacteria which causes clinical mastitis in cattle is S. aureus, which has become resistant to most common antibiotics in veterinary medicine. Therefore, researchers are looking for new methods in the treatment of such infections, in which the pigments of microorganisms fall into this category. The aim of this recent study was to optimize the production of carotenoid pigments by Rhododorula yeast and to investigate its antimicrobial effect on S. aureus isolates To isolate S. aureus, samples were taken from the milk of 100 cows with mastitis and the femA gene was identified for molecular confirmation among suspicious isolates of Staphylococcus by PCR method. Also, the isolate of R. glutinis obtained from one of the samples of mastitis was further examined and used as a sample of yeast producing pigment. The results showed that in addition to S. aureus as one of the causative agents of mastitis in dairy cattle, but also the yeast R. glutinis can be a causative agent of mastitis. The inhibitory effect on the growth of Staphylococcus using the pigment extracted from R. glutinis was also quite evident and more than 80% of S. aureus isolates were sensitive to the pigment at a concentration of 200 μg. R. glutenis inhibits excellent growth in S. aureus as one of the leading causes of mastitis in cows. The cause is the appearance of synthetic antibiotics and the reduction of microbial resistance.

References:

1. Alni, RH., Mohammadzadeh, A., Mahmoodi, P)2018(. Molecular typing of Staphylococcus aureus of different origins based on the polymorphism of the spa gene: characterization of a novel spa type. 3 Biotech. 8, 1-7. https://doi.org/10.1007/s13205-017-1061-6.


2. Ruegg., PL. ) 2017(. 100-Year Review: Mastitis detection, management, and prevention. J Dairy Sci. 100, 10381-10397.


3. Cheng, WN., Han, SG. ) 2020(. Bovine mastitis: risk factors, therapeutic strategies,and alternative treatments A review. Asian Austral J Anim Sci. 33, 1699. https://doi.org/10.5713/ajas.20.0156

4. Derakhshani, H., Fehr, KB., Sepehri, S., Francoz, D., De Buck, J., Barkema, HW., et al)2018(Invited review: microbiota of the bovine udder: contributing factors and potential implications for udder health and mastitis susceptibility. J Dairy Sci. 101, 10605-25. https://doi.org/10.3168/jds.2018-14860


5. Sanna, C., Marras, L., Desogus, A., Marras, B., Montero, N., Bertolino, G., et al. ) 2021(. Evaluation of Rhodotorula spp. contamination in hospital environments. Environ Monit Assess. 193, 1-6. https://doi.org/10.1007/s10661-021-08908-3


6. Enshaeieh, M., Madani, M., Abdoli, A., Nahvi, I., Asgari, F. ) 2016 (. Optimization of SCO and xylitol production in the oleaginous yeast Rhodotorula mucilaginosa. Appl Biol.29, 5-21. https://dx.doi.org/10.22051/jab.2021.4192.0


7. Halasa, T., Huijps, K., Østerås, O., Hogeveen, H. ) 2007(. Economic effects of bovine mastitis and mastitis management: A review. Vet Pathol. 29,, 18-31

 https://doi.org/10.1080/01652176.2007.9695224

8. Tabaee, M. In vitro antibacterial effects of marbofloxacin on microorganisms causing mastitis in cows. J Vet Res.65, 51-55


9. Hernández-Almanza, A., Montanez, JC., Aguilar-Gonzalez, MA., Martínez-Ávila, C., Rodríguez-Herrera, R., Aguilar, CN)2014(. Rhodotorula glutinis as source of pigments and metabolites for food industry. Food Biosci. 5, 64-72. https://doi.org/10.1016/j.fbio.2013.11.007


10. Mehrotra, M., Wang, G., Johnson, WM. ) 2000(. Multiplex PCR for detection of genes for Staphylococcus aureus enterotoxins, exfoliative toxins, toxic shock syndrome toxin 1, and methicillin resistance. J Clin Microbiol. 38,1032-1035. https://doi.org/10.1128/jcm.38.3.1032.1035.2000


11. Khanipour, E., Keramat, J., Shokrani, R. Determination of Optimum Conditions for Carotenoid Extraction from Tomatoes. 11, 289-296


12. Molineri, AI., Camussone, C., Zbrun, MV., Archilla, GS., Cristiani, M., Neder, V., et al. ) 2021(. Antimicrobial resistance of Staphylococcus aureus isolated from bovine mastitis: Systematic review and meta-analysis. Prev Vet Med. 10, 105261. https://doi.org/10.1016/j.prevetmed.2021.105261


13. Moazamian, E., Emami, A. ) 2018(. Antimicrobial effect of Serratia marcescens Pigment on the Multi-Drug Resistance Klebsiella Pneumoniae Isolates from Burn Wounds. Armaghane danesh. 23,499-515.


14. Sharma, N., Singh, N., Bhadwal, M. )2011 (. Relationship of somatic cell count and mastitis: An overview. Asian Austral J Anim Sci. 24, 429-38. https://doi.org/10.5713/ajas.2011.10233.


15. Moslemipur., F, Mostafaloo, Y., Khanahmadi, A. ) 2016(. Survey of conformity between organoleptic and microbial culture techniques to diagnose cows mastitis and antibiogram test in milk of industrial and traditional herds. Res J Anim Sci.1, 51-56.


16. Shokohi, M., AHMADIZADEH, C., Kaveh, A. ) 2018(. Evaluation of bacterial causes of subclinical mastitis in dairy cattle of negine sabze makoo agro-industrial and animal husbandry complex. Vet Clin Pathol. 44, 379-388. https://doi.org/10.30495/JVCP.2020.1873924.1242


17. Novoveská, L., Ross, ME., Stanley, MS., Pradelles, R., Wasiolek, V., Sassi, J-F. (2019). Microalgal carotenoids: A review of production, current markets, regulations, and future direction. Mar Drugs. 17, 640. https://doi.org/10.3390/md17110640.


18. Colasuonno, P., Marcotuli, I., Blanco, A., Maccaferri, M, Condorelli, GE., Tuberosa, R., et al .(2019). Carotenoid pigment content in durum wheat ( Triticum turgidum L. var durum(: An overview of quantitative trait loci and candidate genes. Front Plant Sci. 10, 1347. https://doi.org/10.3389/fpls.2019.01347


19. Martínez, JM., Delso, C., Aguilar, DE., Álvarez, I., Raso, J. (2020). Organic-solvent-free extraction of carotenoids from yeast Rhodotorula glutinis by application of ultrasound under pressure. Ultrason Sonochem.61, 104833. https://doi.org/10.1016/j.ultsonch.2019.104833


20. Yolmeh, M., Hamedi, H., Khomeiri, M. )2016(. Antimicrobial Activity of Pigments Extracted from Rhodotorula glutinis Against Some Bacteria and Fungi. Zahedan J Res Med Sci. 18, e4954. https://doi.org/10.17795/ZJRMS-4954


21. McKeegan, KS., Borges-Walmsley, MI., Walmsley, AR. )2002(. Microbial and viral drug resistance mechanisms. TIM. 10, s8-s14. https://doi.org/10.1016/S0966-842X(02(02429-0.


22. Wang, W., Zhai, Y., Cao, L., Tan, H., Zhang, R. )2016(. Endophytic bacterial and fungal microbiota in sprouts, roots and stems of rice )Oryza sativa L.(. Microbiol Res. 1, 1-8.