Effect of Feeding Different Levels of Protein on Growth of hemolymph protein of Honey Bee (Apis mellifera)

Subject Areas : Journal of Comparative Pathobiology

L. Eskandari ¹ , GH. Ghalamkari ² , , A. Gheysari, ³

1 - MSc of Animal Science, Department of Animal Science, Islamic Azad University, Khorasgan (Isfahan) Branch, Iran
2 - Associate Professor of Department of Animal Science, Islamic Azad University, Khorasgan (Isfahan) Branch, Iran
3 - Associate Professor of Poultry Nutrition, Isfahan Agricultural Research Center, Esfahan, Iran

Received: 2022-02-23 Accepted : 2022-02-23 Published : 2021-08-23

Keywords: Nutrition, Protein, hemolymph, Honeybee,

Abstract :

The present study was conducted to evaluate hemolymph protein changes in the effect of feeding different levels of the protein of honey bee(Apismellifera L.). The experiments carried out with five treatments, including different levels of protein (0, 2.5, 5, 7.5 and 10 percent) and 4 replications in a completely randomized design with four replicates in the incubator condition. poly-acrylamide gel electrophoresis in sodium dodecyl sulfate (SDS)methode was used for the separation of proteins extracted from Hemolymph. Antibacterial peptides expression of defencin and abasin in hemolymph at the age of 12 days between treatments was statistically significant and 10% protein treatment expression showed the highest and control group the lowest value in defencin peptide (2293 units against 1412 units respectively) and about abasin peptide,treatment of 5 % protein showed the greatest amount and control and 2.5% protein treatment showed the lowest value of expression (1970 units against 1257 and zero respectively) (p<0.0001). Totally, the results showed that the profile of protein of hemolymphand of honey bee with feeding different levels of protein changed and improved.

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