Design of a biosensor using antibodies immobilized on the quantum dots and rhodamine attached to citrinin to detect mycotoxin

Subject Areas : Journal of Comparative Pathobiology

بهروز Shojaee Sadi, ¹ (Department of Pathobiology, Faculty of Veterinary Specialized Sciences, Science and Research Branch Islamic Azad University, Tehran, Iran.)
Mansour Bayat ² (Professor of mycology,Department of Pathobiology,Science &b Research Branch,Islamic Azad University,Tehran,Iran)
, P Tajik ³ (Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran)
SJ Hashemi ⁴ (Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.)

Received: 2021-11-03 Accepted : 2021-11-03 Published : 2020-12-21

Keywords: Nano-biosensor, rhodamine, citrinin, Quantum dot,

Abstract :

Citrinine is a nephrotoxic metabolite that damages the kidneys in animals. Therefore, the development of detoxification methods during the food process is important. Quantum devices, using a single source of excitation, are excited by individual wavelengths and produce different emission peaks. In this study, a new bioconjugation-based sensor consisting of quantum dot semiconductor nanocrystals and anti-citrinin antibody was used to determine the amount of citrinin toxins using the FRET (Fluorescence Resonance Energy Transfer) system. Semiconductor nanocrystals or quantum materials (CdTe, CdTe / CdS) were synthesized in the laboratory using reflux method by reducing tellurium powder under a stream of nitrogen gas. The fluorescence intensity of quantum data was determined using a spectrofluorimetric device. Antibody activity in nanobioconjugate was also measured by spectrofluorimetry. The citrinin-albumin conjugate was then attached to the fluorescence compound rhodamine 123, which is a highly affinity immunological reaction between the anti-citrinin-quantum antibody (donor) and the citrinin-rhodamine 123 (receptor) label used as fluorophora. After adding the nanobioconjugated solution to the cuvette, which contains a certain amount of phosphate buffer, citrinin was added to it in different concentrations, and after a few minutes, changes in the intensity of quantum dot fluorescence were observed. A regular decrease in fluorescence intensity was obtained by increasing the citrinin concentration. Due to the collective effects of mycotoxins on human and animal health, it is better to determine the extent of food contamination with mycotoxins, including citrinin, using nanobiosensors as a specific method that results from the use of monoclonal antibodies. Unlike conventional methods such as ELISA and HPLC, these designed nanobiosensors are homogeneous, simple, fast, and inexpensive and do not require much separation or rinsing.

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