The comparison of polymerase chain reaction (PCR) and loop-mediated isothermal amplification (LAMP) methods for detecting Fasciola hepatica in faecal samples of sheeps in Lorestan province

Subject Areas : Journal of Comparative Pathobiology

, S.  Amiri ¹ (.Department of Pathobiology, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran)
bahar  shemshadi ² (Department of Pathobiology, Faculty of Veterinary Medicine, Azad University, Science and Research Branch, Tehran, Iran)
, S  Fallahi ³ (Department of Parasitology and Mycology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran)
, S.  Shirali ⁴ (Department of Biotechnology, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran)

Received: 2021-11-01 Accepted : 2021-11-01 Published : 2020-11-21

Keywords: Sensitivity, Diagnosis, digene, Parasite,

Abstract :

Fascioliasis is a zoonotic disease with long history in Iran. Different methods are used for detection of the disease and one of methods is LAMP. The present study was conducted to compare the LAMP and PCR methods for detection of Fasciola hepatica in fecal samples of sheeps in Lorestan province. During 3 months, 195 samples were collected by classical randomized method. The samples were stored in -20ºC for future investigations by LAMP and PCR. The samples were investigated by LAMP and PCR. The data were analyzed by SPSS software and Chi-square and Kappa coefficient was used. The results showed that LAMP technique showed significant difference with LAMP and detected infected samples (11 samples in LAMP and 7 samples in PCR). The results for kappa showed a strong agreement between the both methods with coefficient of 0.65 and P=0.001). The LAMP method showed higher sensitivity. In sum, LAMP method showed higher sensitivity compared to other methods and can be used for detection of F. hepatica.

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