Survey Genotyping of Animal and Human ‏ ‏‏Klebsiella pneumoniae‎ Isolates using ERIC-PCR and evaluation ‎ of Antibiotic Sensitivity Pattern

Subject Areas : Journal of Comparative Pathobiology

Estabraghi, E., Zahraei Salehi, T.*, Amini, K., Jamshidian, M. . ¹

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Received: 2018-04-13 Accepted : 2018-04-13 Published : 2018-06-22

Keywords: Klebsiella pneumoniae, ERIC-PCR, Antimicrobial resistance,

Abstract :

Klebsiella pneumoniae, is an opportunistic pathogens and cause infections in humans and animals. Drug resistant K. pneumoniae is rising. Therefore, antimicrobial susceptibility testing before prescribing antibiotics, it seems necessary. The aim of present study was to survey typing of clinical and animal K. pneumoniae isolates and evaluation of antibiotic susceptibility. A total 100 clinical and animal K. pneumoniae isolates were collected from Babak city. Antibiotic susceptibility was performed with Kirby-Bauer method according to CLSI guidelines. Then, DNA genomic extraction was done using DNA kit and PCR amplification was performed with ERIC1 and ERIC2 primer. Our results were shown that all strains (100%) were resistant to the ampicillin and amikacin antibiotics. The most and least resistance belong to tetracycline (53 strains; 88.3%) and imipenem (8 isolates; 13.3%), respectively. The results of cluster analysis and drawing dendrogram based on genetic similarities for 100 isolates was separated to seventeen distinct groups. According to our finding indicated an increasing resistance to antibiotics amongst K. pneumoniae. Additionally, the ERIC sequences have a pair of games that contain highly reversed and central reps and are located in the outermost regions of the bacterial genome and have less complexity in determining the genetic diversity of all isolates, but the separation good at the strain level.    

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