Study of rosemary extract on BCL2 and BAX gene expression in canine mammary gland carcinoma cell line (CF41.Mg)

Subject Areas : Journal of Comparative Pathobiology

Shabani, S., Mortazavi, P. . ¹

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Received: 2017-02-12 Accepted : 2017-02-12 Published : 2017-03-21

Keywords: Canine mammary tumor, Cell culture, Rosemary extract, BCL-2, BAX,

Abstract :

Apoptosis is named genetically programmed cell death and have special role in physiological and pathological conditions. BAX protein as a key protein in the apoptosis induced by various factors in the apoptosis pathway acts and BCL-2 have anti-apoptotic effect in response to various stimuli of the mitochondrial apoptosis by preventing the release of cytochrome C applies. In this study, the effect of rosemary extracts on the expression of BCL-2 and BAX genes on breast cancer cells dogs (CF41.Mg). in in vitro examined and the control group (which cell line CF41.Mg without drugs) were compared Cells at doses of 5,10,25,50 and 100 micromole rosemary extract for 24,48 and 72 hours were exposed then by using MTT, the effect of drug on cell survival were analyzed. The tests in which different levels of rosemary extract was added to the cell culture, it was determined that rosemary extract induced cell death after 48 hours’ maximum amount to be allocated. The result suggests that the antitumor activity of the extract after 48 hours at a dose 25 mg/ml is more effective. The results of this study confirm the inhibitory effect of rosemary extract on canine mammary tumor cells (CF41.Mg).