Prevalence of antibiotic resistance genes in Salmonella enteritidis isolated from animal and human and determining their antibiotic resistance patterns

Subject Areas : Journal of Comparative Pathobiology

کیومرث امینی . ¹ (.)

Received: 2016-02-25 Accepted : 2016-02-25 Published : 2016-02-20

Keywords: Salmonella enteritidis, Class I integrons, Antibiotic resistance,

Abstract :

Salmonella is a member of the Enterobacteriaceae family. Infections in humans that are caused by these bacteria, are manifested asgastroenteritis, enteric fever (typhoid or para-typhoid) and septicemia. Class I integrons are the most common integrons found in the different serotypes of Salmonella enterica, that has caused multiple antibiotic resistance and problems in the treatment of infections resulted from these bacteria, in humans and animals. The aim of this study was to determine the prevalence of class I integrons in Salmonella enteritidis strains isolated from human and animal, and their antibiotic sensitivity or resistance. In this study, human)11 samples) and  animal (13 samples) Salmonella enteritidis samples were received from the microbial collection of Islamic Azad University- Science and Research branch- faculty veterinary medicine . Then multiplex PCR experiments were performed to determine the frequency of class I integrons. Finally, agar disk diffusion method was used to determine antibiotic resistance pattern. The results showed that none of the 11 human Salmonella enteritidis strains had the class I integrons and 2 strains (15.3%) out of 13 animal Salmonella enteritidis had the class I integrons. As well 36.3% of human Salmonella enteritidis strains and 30.8% of animal Salmonella enteritidis strains showed multiple antibiotic resistance. Conclusion: In multiple-resistant strains that lacked class I integrons, resistance genes can be located on plasmids, transposons or other integron classes