Phylogenetic analysis of coxiella burnetii isolates from dairy farms in Tehran
Subject Areas : Journal of Comparative Pathobiologyچنگیز احمدیزاده، محمود جمشیدیان، فرهاد موسیخانی . 1
1 - .
Keywords: Coxiella burnetii, Phylogenetic analysis, PCR ,
Abstract :
Q fever is a zoonotic disease with worldwide distribution Caused by a Rickettsia called coxiella burnetii through the mass of aborted fetuses, urine, and feces excreted in the milk of infected animals. Thus, consumption of unpasteurized milk contamination can be a source of infection for humans. The aim of this study phylogenetic analysis Coxiella burnetii was isolated from dairy farms in Tehran After obtaining the desired gene sequence of the bacterium Coxiella burnetii, the sequences obtained were compared with sequences in the gene bank. In this study, a total of 50 dairy farms around Tehran in 150 milk samples were received from the reservoir tank and all the samples using primers specific genes, transposons IS1111a coxiella burnetii, the PCR test were Of the total samples, 18 samples positive coxiella burnetii frequently randomly selected 12 samples were sent for sequencing to Korea Macrogen company. The results showed that cow's milk is one of the potential reservoirs coxiella burnetii in Iran. The result of this comparison showed that the transposons IS1111A Coxiella burnetii gene isolated from milk farms around Tehran with more than99% sequence similarity to the gene bank.