Long-term effects of green tea extract on the damage caused by Doxorubicin in rat bone marrow

Subject Areas : Journal of Comparative Pathobiology

M. Moosavi, ^{1 *} , P. Mortazavi, ^{2 *} , N. Atyabi, ^{3 *} , M.A Porkabireh, ^{4 *}

1 - دانشجوی دامپزشکی، دانشکده تخصصی دامپزشکی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
2 - گروه پاتوبیولوژی، دانشکده تخصصی دامپزشکی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران ، ایران
3 - گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه تهران، تهران ، ایران
4 - گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه تهران، تهران ، ایران

Received: 2014-08-09 Accepted : 2015-01-19 Published : 2015-07-23

Keywords: Doxorubicin, Green Tea, Bone marrow, Rat,

Abstract :

One of the most well known anti-cancer drug is Doxorubicin. However, green tea has manyantioxidant properties. In this study, the long-term effects of green tea on bone marrow lesionscaused by Doxorubicin in rats were studied. 30 adult male Wistar rats were randomly dividedinto 6 groups. Control, LGT (long-term use of 3% green tea extract for 60 days), SHGT (shorttermuse of 3% green tea extract for 10 days), LGT + DXR (long-term use of 3% green teaextract for 60 days with injections IP doxorubicin for 3 days), SHGT + DXR (short-term use of3% green tea extract for 10 days in combination with doxorubicin (IP) injection for 3 days),DXR (doxorubicin injection for 3 days). 24 hours after the last injection of DXR, rats euthanizedand tissue samples were taken for histopathology of the femur. The enzymes catalase andsuperoxide dismutase (SOD) were measured. The histopathology results show that the DXRinduced severe bone marrow suppression that myeloid cells was more involved. SHGT + DXRgroup was similar to DXR, but the cell density was similar to control group. The LGT + DXRcell density was very similar to the control group. There was a small amount of myeloid celldestruction. Measurement of catalase and SOD indicates a significant difference betweentreatment and control groups. Results show that Doxorubicin may cause damage in rat bonemarrow, but long-term use of green tea can reduce the damage caused by doxorubicin.