Identification of mycobacterium avium subsp paratuberculosis in samples tissue ileum of cattle slaughtered in Shahr-e Kord with ziehl neelsen staining and nested PCR method

Subject Areas : Journal of Comparative Pathobiology

حسن  کریمی ¹ (ندارد)
عبدالرسول  نامجو ² (ندارد)
حسن  ممتاز ³ (ندارد)
محمدرضا  نامداری ⁴ (ندارد)

Received: 2011-09-07 Accepted : 2011-11-13 Published : 2012-02-20

Keywords: Johne’s disease, Mycobacterium paratuberculosis, Bovine, Histopathology, Ziehl–&#x0D, &#x0D, Neelsen, s staining, PCR,

Abstract :

Johne s disease is a severe chronic progressive enteritis that infect digestive system ofruminants, domestic animals, wild animals and equine .This study performed due to distinguishM. avium paratuberculosis with nested polymerase chain reaction and Ziehl–Neelsen, s stainingon the ilieum samples of slaughtered cattle in Shahr-e- Kord industrial slaughterhouse. Resultsshowed that respectively 6.6% and 26.67 % of bovine are contaminated with M. aviumparatuberculosis according to histopathology method and nested polymerase chain reaction .Mac nemar statistical test showed that sensitivity of PCR test was more than histopathologicalmethod( P<0.001) . Also 80% total agreement between these two methods and the Kappacoefficients calculated at 0.33 which represent is poor agreement (P<0.01). There wasn’tsignificant deference between, gender, race and age with infection rate ( P>0.05). The resultsshowed that a nested PCR test for diagnosis of infection is high speed and accuracy and in theprimary levels of disease that amount of microorganism is a few we cannot trust to result ofhistopathological method for recognition of Johne‚s disease