Identify of Sarcocytis hominis in cattle carcasses using PCR method

Subject Areas : Journal of Comparative Pathobiology

ابراهیم رحیمی ¹ , عباس دوستی ² , سید رضا حسینی ³ , مجید همایونی ⁴ , رحمان عبدی زاده ⁵

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Received: 2011-12-27 Accepted : 2012-06-08 Published : 2012-08-22

Keywords: Sarcocystis hominis, Cattle, PCR,

Abstract :

A survey was carried out to investigate the occurrence of Sarcocystis spp. and identification ofSarcocystis hominis slaughtered cattle's in Isfahan slaughterhouse. In all, the muscle tissue(cardiac and diaphragmatic muscles) of 768 samples from 384 cattle carcasses were examinedby microscopic method. Based on the microscopic examination the prevalence rate ofSarcocystis cysts in the cattle's were 62.8%. Based on the PCR method, 207 sample from 431infected samples (48.1%), were positive for Sarcocystis hominis. Not significant difference wasobserved between the prevalence in cardiac and diaphragmatic muscles (P>0.05). The resultsindicated that Sarcocytsis cysts in the Isfahan is consumption of under–cooked cattle meat mayresult in Sarcocytsis in humans.