Effect of Cysteamine Supplementation on in vitro Maturation of bovine Oocyte

Subject Areas : Journal of Comparative Pathobiology

رحیم بهشتی ¹ (ندارد)
جمشید قیاسی قلعه کندی ² (ندارد)
آمنه محمدی روشنده ³ (ندارد)

Received: 2011-10-10 Accepted : 2012-04-18 Published : 2012-05-21

Keywords: Bovine oocyte, cysteamine, IVM, anti-oxidant,

Abstract :

This study was aimed at determining the effect of cysteamine supplement during in vitromaturation of bovine oocytes. Cumulus-oocyte complexes (COCs) from abattoir ovaries werewere obtained from local abattoir (Tabriz abattoir, East Azarbaijan, Iran) shortly after slaughterand transported to the laboratory in 0.9% NaCl solution plus 100 IU/ml potassium penicillin G,and 100 μg/ml streptomycin sulfate at 30-35°C, within 2 to 4 h from slaughter. Cumulus-oocytecomplexes (COCs) from abattoir ovaries were matured in vitro in Hepes-TCM 199supplemented with 0.2 mM sodium pyruvate, 1 μg/ml 17-β-estradiol, 10% fetal calf serum(FCS), 0.5 μg/ml bFSH and 0 (control) and 0.1, 0.5 or 1 mM/ml of cysteamine for 24 h. WhenCOCs matured in TCM 199 media with 0.1 mM/ml cysteamine, the rate of maturation wereincreased as compared with control group (46.66 vs 33.33%, respectively). The maturation rateof oocytes in media with 0.5 mM/ml cysteamine (56.55%) was significantly higher than controlgroup (33.33%) (P<0.05). Also, the percentage of unmatured oocytes in 1 mM/ml treatmentgroup lower than other groups (P<0.05). The results demonstrate that cysteamine when presentduring IVM, improved bovine oocyte maturation.