Antibacterial activity evaluation of Ephedra Major Host acetonic, aqueous and alcoholic extracts against standard strains of E. coli, P. aeruginosa, S. aureus and S. pyogenes

Subject Areas : Journal of Comparative Pathobiology

P.  Torabzadeh Khorasani ¹ (ندارد)
پورداد  Panahi, ² (ندارد)
آذر  Sabokbar ³ (ندارد)
علیرضا  Mokhtari ⁴ (ندارد)

Received: 2010-07-26 Accepted : 2010-11-04 Published : 2010-02-20

Keywords: Antibiotics, Folk medicene, Ma hung, Palnt exctract, phytomedicen,

Abstract :

According to WHO reports increase of antibacterial resistance is a growing problem in manycountries. Many efforts have been made to discover new antimicrobial compounds from variouskinds of sources such as micro-organisms, animals, and plants. One of such resources is folkmedicines. Systematic screening of them may result in the discovery of novel effectivecompounds. This study was undertaken to evaluate the growth inhibitory activity of Ephedramajor Host, an important medicinal plant with various biological activities, against pathogenicBacteria involved in common infection.Plant collection was made from district west Tehran,Iran. Extracts were obtained from arial part of plant using ethanol, acetone, methanol and water.Extract was evaporated under vacuum at 60° C. Antibacterial activities of extracts weredetermined by In Vitro bioassays using agar diffusion-method, MIC and MBC against standardstrains of Staphylococcus aureus (ATCC 6538), Pseudomonas aeruginosa (ATCC 27853),Streptococcus pyogenes (PTCC 1447) and E. coli (ATCC 8739). E. coli exhibit sensitive only toacetone extract, Pseudomonas aeruginosa was resistance to water extract. Staphylococcusaureus and Streptococcus pyogenes were sensitive to all tested extracts.