Investigating the effect of Avastin on the apoptosis of HepG2 cells co-cultured on hydrogel containing HUVEC cells through the function of P53 molecular pathway and changes in the level of oxidant oxygen species.
Subject Areas :
Elham Naghimousavighezeljeh
1
,
H Jafari
2
*
,
Zahra Kianmehr
3
1 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 - Department of Biochemistry, Faculty of Biological Science, Islamic Azad University, North Tehran Branch, Tehran, Iran.
Keywords: Antioxidant Enzyme, Hydrogel Scaffold, Cancer, Liver, Apoptosis ,
Abstract :
Hepatocellular carcinoma is the fifth most common cancer worldwide, which is associated with severe complications. In this study, the effect of Avastin drug on the proliferation of HepG2 cells was investigated through the activation of the P53 pathway and the change of the antioxidant content of HepG2 cells cultured on a hydrogel scaffold containing HUVEC cells. In this study, HepG2 cells were cultured on type I collagen hydrogel scaffolds containing HUVEC and after treatment with different concentrations of Avastin, cell viability was measured using the MTT test and the rate of apoptosis using flow cytometry. The level of lipid peroxidation, superoxide dismutase (SOD) enzyme activity, total oxidant status (TOS) and total antioxidant capacity (TAS) were measured using calorimetric method and p53 protein level was measured using immunocytochemistry technique. Examining the results shows that the rate of apoptosis in HepG2 cells co-cultured with HUVEC-Scaffold and Avastin drug is significantly higher than other groups. The results show a significant increase in the amount of total oxidant and malondialdehyde and a significant decrease in the activity of the superoxide dismutase enzyme and total antioxidant capacity in the two groups of HepG2+Scaffold-HUVEC and HepG2+Scaffold treated with Avastin. Avastin can increase the percentage of p53 in HepG2 cells co-cultured with Scaffold and Scaffold-HUVEC. In this study, hydrogel scaffold was used as an option for optimal simulation of the life of cancer cells and it was shown that the drug Avastin has the ability to have a good anti-cancer effect in these structures.
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