Evaluation of the restorative effects of dexamethasone and tacrolimus in improving peripheral nerve lesions in mice

Subject Areas : Journal of Comparative Pathobiology

Sh Safaei ¹ , H Fattahian ^{2 *} , S Hesaraki ³

1 - Department of Veterinary Medicine, Islamic Azad University, Tehran Science and Research Branch, Tehran, Iran
2 - Department of Clinical Sciences, Faculty of Specialized Veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran- Iran
3 - Faculty of Specialized Veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran- Iran

Received: 2024-04-24 Accepted : 2024-08-04 Published : 2024-09-21

Keywords: Peripheral nerves, sciatic nerve, nerve repair, dexamethasone, tacrolimus, SFI.,

Abstract :

Abstract: The purpose of this study was to investigate the regenerative effects of dexamethasone and tacrolimus in improving peripheral nerve lesions in mice. 25 adult male mice were prepared. The rats were divided into 5 groups: sham (without therapeutic intervention), control (injection of distilled water daily until the 28th day after surgery), experiment one (2 mg/kg), experiment two (5 mg/kg tacrolimus), experiment three (2 mg/kg dexamethasone and 5 mg/kg tacrolimus) were divided before the surgery. The rats were evaluated in terms of movement, and the way of walking was evaluated by placing the foot on the paint and walking on the white paper. The use of dexamethasone and tacrolimus significantly decreased the amount of leukocytes. The lowest rate of axonal degeneration was related to the tacrolimus, dexamethasone and tacrolimus + dexamethasone groups, which did not have a significant difference with each other and all caused a significant decrease in axonal degeneration. The lowest SFI was related to dexamethasone group and tacrolimus and dexamethasone group. On the 14th day, the lowest amount of SFI was related to tacrolimus and dexamethasone group. The lowest amount of SFI on day 21 was related to group 4, followed by dexamethasone and tacrolimus groups, which did not differ significantly from each other. The lowest amount of SFI on day 28 was related to sham, 4, dexamethasone and tacrolimus groups. In general, the results of this study showed that the use of the used medicinal combination led to the improvement of peripheral nerve lesions.

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