Molecular investigation of genes (CspA, CspB and Spb1) of Streptococcus echoes isolated from respiratory samples of horses suffering from acute respiratory infection by multiplex PCR method
Subject Areas :
Ali Shamsizadeh Meymandi
1
,
mohammad mazrouei sabadni
2
,
Babak Kheirkhah
3
,
ehsan stabraghi
4
1 - Graduated with professional doctorate in veterinary medicine, Islamic Azad University, Baft branch
2 - Assistant Professor, Department of Internal Medicine, Faculty of Veterinary Medicine, Baft Branch, Islamic Free University
3 - Department of Microbiology, Faculty of Basic Sciences, Azad Basic Sciences and Research University, Kerman, Iran
4 - Department of Microbiology, Faculty of Basic Sciences, Azad Basic Sciences and Research University, Kerman, Iran
Keywords: streptococcus equi, virulence genes (CspA, CspB and Spb1), foal respiratory infection, M-PCR,
Abstract :
Streptococcus equi is an important pathogenic pathogen in horses, which is associated with respiratory tract infections in foals and is also one of the causes of uterine infections in mares. Streptococcus equi can be isolated from various infections in many animals including pigs, sheep, cattle, goats, dogs, foxes, birds, rabbits and guinea pigs. The present study was carried out in a descriptive-cross-sectional manner. It was a research study and the statistical population was among all the horses with upper respiratory infection caused by Streptococcus equi (40 cases) in the stables of Kerman province. M-PCR reaction was performed on all 40 isolates of Streptococcus equi isolated from horses. The results showed that the presence of CspA, CspB and Spb1 genes in the isolates under study was confirmed by Multiplex-PCR method. CspA and CspB genes were present in all isolates (100%), but Spb1 gene was not found in any of the isolates.Genetic diversity shows that three genes, CspB, CspA and Spb1, are more effective than other genes in bacterial virulence and virulence, and as an important factor for investigating disease and disease susceptibility, it may also be a potential target for vaccine development in streptococci.
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