Stereological and biochemical study of effects of thiamine and ZnO NPs on cecum after l-arginine-induced experimental pancreatitis in rats

Subject Areas : Anatomy

Rahmat Allah Fatahian Dehkordi ¹ , Soren Nooraei ² , Alborz Yadolahi ³ , Parsa Rahmani ⁴

1 - Department of Anatomical Sciences, Department of Basic Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
2 - Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Shiraz, Iran
3 - Department of Anatomical Sciences, Department of Basic Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
4 - Department of Anatomical Sciences, Department of Basic Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran

Received: 2024-01-03 Accepted : 2024-04-03 Published : 2024-11-03

Keywords: Pancreatitis, Rats, Histomorphometry, Biochemical evaluation, ZnO NPs,

Abstract :

Acute pancreatitis (AP) is a critical disease with a high mortality rate due to the necrosis of the pancreas and other organs. We aimed to study the effects of thiamine and ZnO nanoparticles (NPs) in treating AP. Fifty male rats, with an average weight of 28 to 32 grams, were randomly divided into five groups: 1: Control group, 2: L-arginine group (300 mg/100g), 3: Thiamine group (70 mg/kg), 4: ZnO NPs group (1 mg/kg), 5: Combination treatment group: L-arginine (300 mg/100g) + thiamine (70 mg/kg) + ZnO NPs (1 mg/kg). After stereological examination of tissue sections and measurement of biomarkers, the data were analyzed at a significance level of p≤ 0.05. According to the results, in the L-arginine treatment group, the thickness of the mucosal, muscular, and adventitial layers decreased in comparison to the total thickness (p≤ 0.05). Additionally, values of the surface area and density of the mucosal and muscular layers decreased, as well as the total values of these, compared to the control group. The mentioned parameters increased in the combination treatment group. Evaluation of cholesterol showed a significant increase between the control group and groups 4 and 5. Analysis of ALT and AST factors indicated a significant increase in the level of AST in L-arginine group compared to the control group, and a significant decrease in the level of ALT in comparison between the thiamine and ZnO NPs groups and the L-arginine group. Based on the results of the present research, thiamine and ZnO NPs manifested enhancing effects on the values of thickness, surface area, volume density, and level of ALT.

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