The effect of L-carnitine on ram epididymal sperm motility parameters during preservation in HTF medium with CASA
Subject Areas : Theriogenology and Reproductive Medicine
1 - Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran
2 - Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran
Keywords: Ram, Sperm, L-carnitine, HTF, CASA,
Abstract :
L-carnitine, an endogenous amino acid, is highly effective in preventing programmed cell death. As an antioxidant, it also protects DNA structure against reactive oxygen species and increases the activity and amount of antioxidant enzymes. In this study, 20 pairs of mature ram testicles were collected immediately after Islamic slaughter from the Urmia industrial slaughterhouse. The samples were transferred to the faculty laboratory in a cool box. After cleaning and drying the testes from blood, a cut was made in areas without capillaries on the tail of the epididymis. The sperm were transferred to microtubes containing HTF (Human Tubal Fluid) culture medium with 10% bovine serum albumin. After preparing a dilution of 30 to 50 million sperm per ml of HTF, five levels of L-carnitine (0, 2, 4, 6, 8, 10 mM) were added to the medium. The samples were then kept in a refrigerator at 5 degrees Celsius for up to 36 hours. At 1, 12, 24, and 36 hours, the sperm motility pattern was evaluated with CASA. Statistical results showed that L-carnitine at levels of 6, 8, and 10 mM and at 24 and 36 hours caused a clear and significant increase in the motility indices of ram epididymal sperm compared to the control group (p<0.05).
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