Evaluation of antibiotic resistant and molecular identification of Escherichia coli isolates from poultry colibacillosis in Tabriz city during 2020
Subject Areas : Journal of Basic and Clinical Veterinary Medicine
1 - Department of Microbiology, Maragheh Branch, Islamic Azad University, Maragheh, Iran
Keywords: poultry, Escherichia coli, Antibiotic resistance, Colibacillosis,
Abstract :
This study conducted to evaluation of antibiotic resistant and molecular identification of Escherichia coli isolates from poultry colibacillosis in Tabriz city during 2020. Forty-six Escherichia coli samples from colibacillosis cases of 10 aviculture in Tabriz city, which had been previously isolated and detected by microbiological and biochemical methods, were identified molecularly with 16s rRNA primers by PCR method. To determine the pattern of antibiotic resistance of isolates, common antibiotics available in the market were used by Kirby-Bauer method. Of 46 Escherichia coli samples tested, 40 isolates (86.9%) were identified as Escherichia coli. All strains of Escherichia coli (100%) were resistant to ampicillin. The most strains were resistant to ceftriaxone (75%), neomycin (70%) and nitrofurantoin (70%). Twelve Escherichia coli strains (30%) were resistant to all of tested antibiotics. It can be concluded that the antibiotic resistance of Escherichia coli isolates to common antimicrobial drugs used in the poultry industry is high. So, implementation of national monitoring programs is strongly needed for antimicrobial resistance and rational use of antibiotics.