Fabrication of choline oxidase enzyme-based nanobiosensor for the detection of diazinon and comparing its performance with the high performance liquid chromatography

Subject Areas :

اقدس بنائی ¹ , ناهید پارسافر ² , جلیل بدراقی ³

1 - استادیار، گروه پژوهشی فیزیک، پژوهشکده علوم پایه کاربردی جهاددانشگاهی، دانشگاه شهیدبهشتی، تهران، ایران
2 - مربی پژوهش، گروه پژوهشی فیزیک، پژوهشکده علوم پایه کاربردی جهاددانشگاهی، دانشگاه شهیدبهشتی، تهران، ایران
3 - استادیار، گروه پژوهشی فیزیک، پژوهشکده علوم پایه کاربردی جهاددانشگاهی، دانشگاه شهیدبهشتی، تهران، ایران

Received: 2017-12-17 Accepted : 2018-05-16 Published : 2019-02-20

Keywords: diazinon, HPLC, NanoBiosensor, Cholineoxidase enzyme, Carboxylated carbon nanotube,

Abstract :

Organophosphorus compounds enter the environment naturally or after human use as pesticides or chemical fertilizers, and ultimately enter the food chain of organisms. Due to their undesirable effects, it is absolutely necessary to detect these toxins at very low concentrations before they enter the life cycle. Biosensors are suitable tools for detecting these toxins. In this study, a mono-enzyme biosensor based on inhibiting the cholineoxidase enzyme with its immobilization on a modified electrode with carboxylate carbon nanotubes was fabricated and in the presence of 1mM enzyme substrate )choline chloride(, the minimum detection limit for diazinon was obtained (0.56 μM( and two linear ranges (1.5- 2.4 μM and 5.5-17.5 μM( was observed. The detection of low concentrations of diazinon by this biosensor and high performance liquid chromatography )HPLC( were compared. The result of the biosensor had 3.5% error relative to HPLC

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