The Production of Zerumbone in Adventitious Roots Culture of Zingiber zerumbet Smith

Subject Areas : Biochemistry

Mahanom Jalil ¹ (Centre For Foundation Studies in Science, Universiti Malaya, 50603 Kuala Lumpur, Malaysia.)
Nurul Alwakil ² (Institute of Biological Science, Universiti Malaya)
Boon Chin Tan ³ (Centre of Biotechnology for Agriculture Research (CEBAR), Universiti Malaya, 50603 Kuala Lumpur, Malaysia.)
MOHAMAD SUFFIAN MOHAMAD ANNUAR ⁴ (Institute of Biological Sciences, Faculty of Science, Universiti Malaya, 50603 Kuala Lumpur, Malaysia.)
Norzulaani Khalid ⁵ (Faculty of Art and Sciences, International University of Malaya-Wales, 50480 Kuala Lumpur, Malaysia.)

Received: 2023-04-25 Accepted : 2024-04-24 Published : 2024-04-21

Keywords: herbs, lempoyang, , bioactive compounds, root culture,

Abstract :

Root cultures were established through adventitious roots obtained either from direct or indirect organogenesis. The frequency of root response, number of roots per explant, root length, and zerumbone production were influenced by the concentrations and the types of auxins, initial root inoculum and the strength of the basic Murashige and Skoog (MS) salt in the culture media. It was crucial to decide the type of root explant and optimum media that supported both growth and bioactive compound production in the root cultures. In our study, we found that there was a noncorrelation in the optimised media for growth and zerumbone production in the root cultures of medicinal ginger Zingiber zerumbet Smith. Full strength (MS) medium was the optimum media for specific growth rates whereas zerumbone accumulation was higher in half strength MS medium for adventitious roots from direct (AdRD) and indirect (AdRId) organogenesis. AdRD was chosen over AdRId although the specific growth rate achieved was higher in the latter (7.2 x 10-2µ) than the former (5.5 x 10-2µ) based on the zerumbone accumulation performance. Subsequently, these AdRD root cultures were elicitated with methyl jasmonate which showed ten-folds increase in zerumbone production than the controls. This study could provide a scalable protocol for the production of zerumbone from adventitious root culture in the future.

References:

Akhtar, N., I. Jantan, L. Arshad, M. A. Haque, 2019. Standardized ethanol extract, essential oil and zerumbone of Zingiber zerumbet rhizome suppress phagocytic activity of human neutrophils. BMC Complementary & Alternative Medicine 19(1): 331.
Anbazhagan, V. R., C. H. Ahn, E. Harada, Y. S. Kim, Y. E. Choi, 2008. Podophyllotoxin production via cell and adventitious root cultures of Podophyllum peltatum. In Vitro Cell Dev Biol - Plant 44(6): 494-501.
Ata, N., N. A. Yusuf, B. C. Tan, A. Husaini, Y. M. Yusuf, N.A. Majid, N. Khalid, 2015. Expression profiles of flavonoid-related gene, 4 coumarate: coenzyme A ligase, and optimization of culturing conditions for the selected flavonoid production in Boesenbergia rotunda. Plant Cell Tissue and Org Cult. 123: 47-55.
Baque, M. A., E. J. Hahn, K. Y. Paek, 2010. Induction mechanism of adventitious root from leaf explants of Morinda citrifolia as affected by auxin and light quality. In Vitro Cell Dev Biol – Plant 46: 71-80.
Betsui, F., N. Tanaka-Nishikawa, K. Shimmomura, 2004. Anthocyanin production in adventitious root cultures of Raphanus sativus L. cv. Peking Koushin. Plant Biotechnol. 21: 387-391.
Chin, W. Y. W., M. S. M. Annuar, B. C. Tan, N. Khalid, 2014. Evaluation of a laboratory scale conventional shake flask and a bioreactor on cell growth and regeneration of banana cell suspension cultures, Sci Hort. 172: 39-46.
Chodisetti, B., K. Rao, G. Suryakala, G. Archana, 2015. Gymnemic acid enhancement in the suspension cultures of Gymnema sylvestre by using the signaling molecules-methyl jasmonate and salicylic acid. In Vitro Cell Dev Biol – Plant 51: 88-92.
Cui, X. H.,D. Chakrabarty, E.J. Lee, K.Y. Paek, 2010. Production of adventitious roots and secondary metabolites by Hypericum perforatum L. in a bioreactor. Bioresour Technol. 101: 4708-4716.
Cui, X., H. N. Murthy, J. Zhang, 2020. Effect of nutritional factors on the accretion of secondary metabolites in Malaysia ginseng adventitious roots culture. Plant Biotechnol Rep. 14: 381-386.
Cusido, R. M., J. Palazón, M. T. Pinől, M. Bonfill, C. Morales, 1999. Datura metel: In vitro production of tropane alkaloids. Planta Med. 65(2): 144-148.
Fisher, D. B. 1968. Protein staining of ribboned epon section for light microscopy. Histochemie 16(1): 92.
Geiss, G., L. Gutierrez, C. Bellini, 2009. Adventitious root formation: New insights and prespectives, In: Root Development. T. Beekman, ed. (Oxford: Wiley-Blackwell) 376.
Girisa, S., B. Shabnam, J. Monisha, L. Fan, C. E. Halim, F. Arfuso, K. S. Ahn, G. Sethi, A. B. Kunnumakkara, 2019. Potential of zerumbone as an anti-cancer agent. Molecules (Basel, Switzerland) 24(4): 734.
Gómez-Aguirre, Y. A., A. Zamilpa, M. González, G. Trejo-Tapia, 2012. Adventitious root cultures of Castilleja tenuiflora Benth as a source of phenylethanoid glycosides. Ind Crops and Prod 36: 188-195.
Holopainen, J. K., V. Virjamo, R. P. Ghimire, J. D. Blande, R. Jukunen-Tiitto, M. Kivimaenpaa, 2018. Climate change effects on secondary compounds of forest trees in the forest trees in the Northern Hemisphere. Front. Plant Sci. 9, Article 1145.
Jalil, M., M. S. M. Annuar, B. C. Tan, N. Khalid, 2015. Effect of selected physicochemical parameters on zerumbone production of Zingiber zerumbet Smith cell suspension culture. Evident-Based Complementary and Alternative Medicine:1-7.
Jalili-Nik, M.,M. M. Sadeghi, E. Mohtashami, H. Mollazadeh, A. R. Afshari, A. Sahebkar, 2020. Zerumbone Promotes Cytotoxicity in Human Malignant Glioblastoma Cells through Reactive Oxygen Species (ROS) Generation. Oxidative medicine and cellular longevity, 2020: 3237983.
Kannan, N., M. Manokari, S. M. Shekhwat, 2020. Enhanced production of antraquinones and phenolic compounds using chitosan from the adventitious roots culture of Morinda coreia Buck. And Ham. Industrial Crops and Products 15(7): 112321.
Lee, E. J., K. Y. Paek, 2012. Enhanced productivity of biomass and bioactive compounds through bioreactor cultures of Eleutherococcus koreanum Nakai adventitious roots affected by medium salt strength. Ind Crop and Product 36: 460-465.
Li, S. W., L. Xue, S. Xu, H. Feng, L. An, 2009. Mediators, genes and signaling in adventitious rooting. Bot Rev. 75: 230-247.
Murashige, T., F. Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15: 473-479.
Nagella, P., H. M. Murthy, 2010. Establishment of cell suspension cultures of Withania somnifera for the production of withanolide A. Bioresource Technol. 101(17): 6735-6739.
Oh, T. I., H. J. Jung, Y. M. Lee, S. Lee, G. H. Kim, S. Y. Kan, H. Kang, 2018. Zerumbone, a Tropical Ginger Sesquiterpene of Zingiber officinale Roscoe, Attenuates α-MHS-Induced Melanogenesis in B16F 10 Cells. Int J Mol Sci 19(10): 3149.
Praveen, N., S. H. Manohar, P. M. Naik, A. Nayeem, H. H. Jeong, H. N. Murthy, 2009. Production of andrographolide from adventitious root cultures of Andrographis paniculate. Curr Sci. 96: 694-697.
Ramachandra, R. S., G. A. Ravishankar, 2002. Plant cell cultures: Chemical factories of secondary metabolites. Biotechnol Adv. 20: 101-153.
Saiman, M. Z., N. R. Mustafa, A. E. Schulte, R. Verpoorte, Y. H. Choi, 2012. Induction, characterization, and NMR-based metabolic profiling of adventitious root cultures from leaf explants of Gynura procumbens. Plant Cell Tiss Organ Cult. 109: 465-475.
Sarin, R. 2005. Useful Metabolites from Plant Tissue Cultures. Biotechnology 4(2): 79-93.
Sivakumar, G. 2006. Bioreactor technology: a novel industrial tool for high-tech production of bioactive molecules and biopharmaceuticals from plant roots. Biotechnol J. 1: 1419-1427.
Sivanesan, I., B. R. Jeong, 2009. Induction and establishment of adventitious and hairy root cultures of Plumbago zeylanica L. African J Biotech. 8(20): 5294-5300.
Sudha, C. G., S. Seeni, 2001. Establishment and analysis of fast-growing normal root culture of Decalepis arayalpathra, a rare endemic medicinal plant. Curr. Sci. 81: 371-374.
Wu, C. H., Y. H. Dewir, E. J. Hahn, K. Y. Paek, 2006. Optimization of culturing conditions for the production of biomass and phenolics from adventitious roots of Echinacea angustifolia. J Plant Biol. 49: 193-199.
Yusuf, N. A., M. S. M. Annuar, N. Khalid, 2013. Physical stress for overproduction of biomass and flavonoids in cell suspension cultures of Boesenbergia rotunda. Acta Physiol Plant. 35: 1713-1719.
Zhang, C. H., J. Y. Wu, G. Y. He, 2002. Effects of inoculum size and age on biomass growth and paclitaxel production of elicitor treated Taxus yunnanensis cell cultures. Appl Microbial Biotechnol. 60(4): 396-402.
Zhang, Z., Z. Yu, Z. Jin, J. Liu, Y. Li, 2013. Liquid culture of adventitious roots is a potential alternative to field cultivation for Psammosilene tunicoides, a rare and endangered endemic medicinal plant. Advance J Food Sci and Tech. 5(2): 127-131.